Ridgeline Forensics notes
forensic Science – PBL Curriculum:
Ch. 1 – Intro to forensic science:
- Purpose of this unit:
- What is forensic science?
- The application of science to law – the application of science to the criminal and civil laws that are enforced by police agencies in a criminal justice system.
- What is forensic science?
- What does it encompass?
- Encompasses criminalistics, digital and multimedia sciences, engineering science, general, jurisprudence, odontology, pathology/biology, physical anthropology, psychiatry/behavioural sciences, questioned documents, toxicology, and even more!
- What is a forensic scientist?
- An individual that acquires physical evidence at a crime scene, or works in a laboratory to decipher what the evidence is.
- What they are not is someone who puts the pieces of the puzzle together to form what may have happened at the crime scene – that is for the detective to do.
- History of forensic science
- 3rd-century Chinese manuscript Yi Yu Ji (A Collection of Criminal Cases)
- What did this manuscript have to do with forensic science?
- A Chinese coroner determined that a man was killed prior to being burned using pigs to look for ash in the mouth.
- What did this manuscript have to do with forensic science?
- 3rd-century Chinese manuscript Yi Yu Ji (A Collection of Criminal Cases)
- A Treatise on Forensic Medicine and Public Health by Francois-Emanuel Fodere (French physician)
- What did this Treatise provide scientists?
- A physician’s guide to the workings of the human body – 1798
- What did this Treatise provide scientists?
- Chemist Carl Wilhelm Scheele (Sweden)
- What did Dr. Scheele produce?
- First successful test for determining arsenic in a person’s blood
- Father of Toxicology – Mathieu Orfila (Spain)
- When and why was the first toxicologist used in a trial?
- The first treatise on the detection of poisons and their effects on animals. First used in 1839 by Scottish chemist James Marsh on the detection of arsenic in a victim’s body.
- When and why was the first toxicologist used in a trial?
- Alphonse Bertillon’s system vs fingerprinting (describe the history and contributors of both)
- What was the Bertillon system? What did it measure?
- 1879 Alphonse’s system of anthropometry was to make several measurements of a criminal (head circumference, arm length, leg length, overall height, ability to bend at the waist, etc.
- When did fingerprinting start being used as a way to identify individuals?
- Fingerprints supplanted this system early in the 1900s as it was a more reliable way to identify individuals.
- What was the reason for the change from the Bertillon system to using fingerprints in the United States?
- The Bertillon system was not dependable, there are many people who have the exact same measurements, but no one has the same fingerprint
- Albert S. Osborn and his book “Questioned Documents”
- What does this book discuss?
- A book still considered a primary reference for document examiners; developed the fundamental principles of document examination and was responsible for the acceptance of documents as scientific evidence by the courts.
- What does this book discuss?
- Is this book still used by forensic scientists today?
- Yes
- Edmond Locard and Locard’s exchange principle
- Who was Edmond Locard?
- Locard asserted that when two objects came into contact with each other, a cross-transfer of materials occurred. He believed that every criminal can be connected to a crime by dust particles carried from the crime scene. He pioneered the way for crime laboratories to be established in police departments after WWI.
- What is Locard’s exchange principle?
- Every contact by a criminal leaves a trace
- Who was Edmond Locard?
- How/where did Edmond Locard set up the first “crime lab”?
- He pioneered the way for crime laboratories to be established in police departments after WWI.
- Modern-day advances (such as DNA, entomology, and others?)
- When was DNA first used to solve a criminal case and who did it help convict?
- DNA typing was first used in 1986 in a criminal case to convict Colin Pitchfork for the murder of 2 young English girls.
- Entomology is the study of bugs and how they affect a decaying body
- The development of the microscope was huge
- The invention of the comparison microscope for ballistics
- When was DNA first used to solve a criminal case and who did it help convict?
- What is entomology?
- Entomology is the study of bugs and how they affect a decaying body
- How is entomology used in forensic science?
- It is used to tell how long ago a person died and how
- How did the invention of the microscope aid in the development of Forensic Science?
- It allowed scientists to view DNA molecules and smaller objects that could be important when solving a case
- How are crime labs “run” today (public vs. private)
- Public – run by the state or local governments, limited by funds, used only for criminal cases
- Private – specialised and can work on both criminal and civil cases
- Court cases that set the standards for allowing forensic evidence into the courtroom. This is your first Capstone Project to complete. Please see the additional handout for this assignment.
- Give a brief (2-3 sentences) description of each case.
- What did each of the above cases do for presenting forensic evidence in the courtroom?
- What standard does Federal Rule 702 set forth?
- 1923 Frye v. the United States – (Required)
- What was this case about?
- What did this case add to the legal presentation of forensic evidence in the courtroom?
- Did NOT allow the “lie detector test” into evidence as it was not a “generally accepted” test in the scientific community.
- Federal Rules of Evidence (particularly rule 702) – (Required)
- What was this case about?
- A witness who is qualified as an expert by knowledge, skill, experience, training, or education may testify in the form of an opinion or otherwise
- What qualifies a person to be an “expert” in their field enabling them to testify in court?
- 1993 Daubert v. Merrell Dow Pharmaceuticals, inc. – (Required)
- What was this case about?
- Overturned the Frye test and set recognized new guidelines for what kind of scientific evidence would be admissible in court – set the courts up as “gatekeepers” of how scientific evidence would be allowed in courts.
- What was this case about?
- How did this ruling affect the Frye rule?
- How did this alter the way courts allow access to forensic evidence in the courtroom?
- United States v. Starzecpyzel – (Optional)
- What was this case about and how was evidence allowed into the courtroom?
- This was about forgery and expert testimony was allowed under rule 702 but not under Daubert
- What was this case about and how was evidence allowed into the courtroom?
- Kumho Tire v. Carmichael – (Required)
- What was this case about?
- The Daubert “gatekeeping” not only applied to scientific expert testimony but to all expert testimony – Rule 702 does not distinguish between scientific knowledge and technical or other specialised knowledge, but makes clear that any such knowledge might become the subject of expert testimony,
- What was this case about?
- What/who qualifies as an expert witness?
- United States v Prime – (Optional)
- What was this case about?
- Fraud case (handwriting) handwriting experts are not part of the scientific community but ARE part of the “technical” community thus their testimony would be allowed in court and could be cross examined.
- What was this case about?
- What is the difference between the scientific community and the “technical” community?
- Coppolino v State (of Florida) – (Required)
- What was this case about?
Coppolino was tried and charged for the murder of his wife
- How was this evidence allowed in court if there had not been “general acceptance” of this type of test?
- A completely novel procedure for detecting succinylcholine chloride was devised and allowed in court.
- Casey Anthony – CSI Effect? Case study
- Casey Anthony trial – https://www.crimemuseum.org/crime-library/famous-murders/casey-anthony-trial/
- Questions:
- How long, and why did Casey Anthony wait to report her daughter missing?
- Questions:
- Casey Anthony trial – https://www.crimemuseum.org/crime-library/famous-murders/casey-anthony-trial/
30 days
- What evidence was presented against Casey Anthony?
There was hair and fibres found in the trunk of her car, grandpa was missing tape
- How was Casey Anthony acquitted of the murder of her daughter?
- What is the CSI effect? –
The CSI effect is because tv has shown us we can have a crime scene and solve it in an hour that it happens like that in real life
- Did the CSI effect have an effect on the Casey Anthony trial? – https://lawrecord.com/2011/08/11/the-case-of-casey-anthony-defending-the-american-jury-system/
- Caylee’s law – what is it and is it a law in AZ.?
Caylee’s law says that if you’re a parent and your child goes missing you have to report it. Arizona does not have it
https://www.alcatrazeast.com/crime-library/criminal-law/caylees-law/
- Investigate: https://www.crime-scene-investigator.net/becomeone.html
- Investigate one or two of the job posting that you have an interest in and do a writeup of why it interests you (something other than pay/income), job description, job growth in that particular area,
- Click on the article link to “Duty Description for the Crime Scene Investigator” by Mike Byrd.
- How should a crime scene be processed (what are the steps in detail)?
- Shirt examination lab – need shirt(s) with 2 types of hair on it – teach how to make a druggist fold and how to properly collect evidence.
Ch. 2 – The crime scene
- Purpose of this unit:
- What are the 7 S’s of crime scene investigation?
- Secure the scene
- Separate the witnesses
- Scan the scene
- photograph the scene
- Sketch the scene
- Search for evidence
- Secure and collect the evidence
- What are the 7 S’s of crime scene investigation?
- Secure and preserve the crime scene – who, how, how big of an area is secured, witnesses, etc.
the first responding officer and his first priority is the victim and those that need medical attention. he’s got to keep the general public out
- Record the crime scene – who, how (what is the process)
3 parts:
Photograph the scene as quickly as possible
rough sketch- contains an accurate picture of the dimensions and objects in the scene, does not need to be neat, needs to have a north south east, and west; just needs to be accurate
Final sketch: a neat sketch of the crime scene: they can capture things some people may not have noticed
Notes: Keeping track of people who entered and exited the scene, notes need to be detailed enough to look back on months or years later
- Conducting a systematic search – who, how (types of patterns), why, what tools might be used
The kind of crime
Where the crime takes place
How big the crime scene is
grid searches, line searches, spiral searches, rays search, quadrant search
- Collecting, Handling, and Packaging physical evidence; Collecting, Handling, and Packaging of biological evidence
- What is the Chain of Custody and how is it maintained?
- Obtain standard/reference samples
- JonBenet Ramsey – https://www.crimemuseum.org/crime-library/cold-cases/jonbenet-ramsey/
- Crime scene – https://www.pinterest.com/OsnapitzLibbyy/jonbenet-crime-scene-photos/
- Crime scene – https://www.thecrimemag.com/a-heartbreaking-look-back-at-jonbenet-ramseys-murder/
- Questions:
- Describe the crime scene.
- Questions:
- How was JonBenet eventually found?
- How would you describe the police response (give examples)?
- How was the evidence handled?
- How was the search of the premises (the house and yard) handled? Give specific examples.
- Was the chain of custody kept intact or broken? Tell me why you think so.
- How could the police have done a better job at securing the crime scene? Give specific examples.
- Scott Peterson Crime – https://www.biography.com/news/scott-peterson-laci-murder-trial-timeline-facts
- Questions:
- What/where was the crime scene for the Scott Peterson case?
- The Scott Peterson case was where Scott was tried for the murder of his wife and unborn son
- The crime scene was off the shoreline of the San Francisco bay
- What/where was the crime scene for the Scott Peterson case?
- Questions:
- What evidence was found at the crime scene?
- A single hair that came from Laci was stuck to pliers on Scotts boat
- How did not having the body of Laci Peterson affect the initial investigators?
- How did the investigation change once the body of Laci Peterson washed up on the shore?
- At first, it was just a missing person case, they couldn’t tell if it was a homicide until they had bodies.
- What evidence was presented at the trial?
- Scott sold his wife’s car before she was killed
- He refused to take a polygraph
- As a crime scene specialist, how would you secure this type of crime scene?
I would take photos of every angle and object there, collect the foetal remains to send to the lab, take a rough sketch of the crime scene, and collect DNA samples from both the wife and the unborn child.
- What are the differences between the JonBenet and Scott Peterson cases?
- Rough sketch and finally cleaned up a sketch of a crime scene – Barbie doll set with a couch, tv, rug, bookshelf, etc.
Chapter 3: physical evidence
- Purpose of this unit: explain difference between identification and comparison of physical evidence
- Common types of physical evidence:
- almost anything can be physical evidence.
- you cannot rely on a list of categories.
- Physical evidence is used to be collected and analyse
- Common types of physical evidence:
- Documents: any handwriting or typewriting
- blood, semen, saliva- liquid or dried, is it human or animal?
- Drugs:any substance in violation of laws regulating sale, manufacture, and distribution
- Explosives: any device containing an explosive charge as well as objects that are suspected to contain residues of explosive
- Fibres: any natural or synthetic fibre whose transfer may be useful in establishing relationship between objects/people
- fingerprints: all prints of this nature hidden (latent) and visible
- firearms and ammunition: any firearm, as well as discharged or intact ammunition
- glass: any glass particle or fragment that may have been transferred to a person or object during the rime
- hair: any animal or human hair present that could link a person to a crime
- impressions: markings, shoe prints, depressions in soft soils, and all other forms of tracts
- organs and physiological fluids: body organs and fluids are submitted for analysis to detect possible existence of drugs and poisons
- paint: any paint, liquid or dried, that may have been transferred from the surface of one object to another during commission of the crime
- petroleum and products: any petroleum products removed from suspect or crime scene
- plastic bags: disposable polyethylene bag such as a garbage bag may be evidence in homicide or drug sale
- plastic, rubber, and other polymers:remnants recovered at the crime scenes may be linked to object recovered
- power residues: any item suspected of containing powder residues resulting from the discharge of a firearm
- Serial numbers: category includes all stolen property submitted to lab for restoration of erased ID numbers
- soil and minerals: all items containing soil or minerals that could link person/object to particular location
- tool marks: contains any object suspected of containing impression of another object that served as a tool in the crime
- vehicle lights: examination of vehicle lights and tail lights is normally conducted to determine whether a light was off or on during time of impact
- wood and other vegetative matter: any fragments of wood, sawdust, shavings or vegetative matter discovered on clothing, shoes, or tools that be a link to person/object linked to a crime location
- Identification and comparison of physical evidences:
- examination is undertaken for identification or comparison purposes
- identification: determination of the physical or chemical identity of substance with as near absolute certainty as existing analytic techniques will permit
- Identification: the object of identification is to determine the physical or chemical identity with as near absolute certainty as existing analytical techniques permit
- process of identification first requires adoption of testing procedure that give characteristic results for specific standard materials
- Second identification:
- once test results established they will be permanently recorded and used repeatedly to prove identity of materials
- identification also requires that the number and type of tests needed to identify a substance be sufficient to exclude all other substances
- comparison analysis:
- subjects specimen and a standard/reference specimen to the same tests and examinations for ultimate purpose of determining whether or not they have a common origin
- 2 step procedure- 1st:
- Crime laboratory frequently requested to identify the chemical composition of illicit drugs. May be asked to identify gasoline in residues covered from debris of fire
- Identification of blood,semn, hair, or wood are included for determination for species origin
- Comparative analysis has important role of determining whether or not suspect and reference specimen have common origin
- both standard/reference and suspect specimen are subject to same tests
- Comparison: Forensic comparison is two step procedure:
- Combination of select properties are chosen from suspect and the reference specimen for comparison
Once examination completed, forensic scientist must be prepared to render conclusion with respect to origins
- 2nd
- individual vs class characteristics
- individual characteristics: Evidence that can be associated to a common source with a extremely high degree of probability is said to possess individual characteristics
- It isn’t possible to state with mathematical exactness the probability that specimens are of common origin
- it can only be concluded that this probability is so high as to defy mathematical calculations or human comprehension
- Individual character examples” comparison of handwriting, comparison of striation markings, comparison of two fingerprints, etc
- class characteristics : Evidence associated only with a group that is said to have class characteristics- I.e blood types
- individual characteristics: Evidence that can be associated to a common source with a extremely high degree of probability is said to possess individual characteristics
- significance of physical evidence
- probability is a determining factor. high diversity of class evidence in environments makes their comparison very significant in context of criminal investigation
- Role of probability: To comprehend evidential value of a comparison, one must appreciate role that probability has in ascertaining the origins of two or more specimens
- Probability is the frequency of occurrence of an event
- Assessing values of: weakness of forensic science: inability of the examiner to assign exact or even approximate probability values to the comparison of most class physical evidence-very few statistical data from which to derive this info, gathering this kind of info is increasingly elusive. Primary endeavour of forensic scientists must be to create and update statistical database for evaluating the significance of class physical evidence
- Cautions and limitations:
- most items of physical evidence retrieved at crime scenes cannot be linked definitively to a single person or object
- two or more objects found at crime scene- chances are they came from the same source
- evidential variations are not the same as natural: natural- occurs in nature evidential: does not occur in nature
- Forensic database
- Fingerprints: integrated automated fingerprint identification system (IAFIS)
- DNA: combined DNA index system, (CODIS)
- Troy Graves- The Center City Rapist
- ballistics/guns/handguns: National Integrated Ballistics information network (NIBIN)
- Others? : PDQ- International forensic automotive paint data query- contains chemical and colour info pertaining to original automotive paint
- Shoe Prints: Shoeprint image capture and retrieved (SICAR)
- Crime scene show prints known as SICAR:
- National missing and unidentified persons system (NAMUS):
- Amanda Knox
- who all were accused of the crime?
- Knox, Sollecito, Lumumba, and Guede
- What “evidence” did they have against amanda?
- there was no evidence against her
- They found Knox’s DNA on the handle of the knife and kerches on the blade. There was also a bloody footprint on a bathmat, bra clasp, and a broken window.
- She was guilty because she showed no remorse, went shopping after the murder, had sex with her boyfriend after and smoked weed, and lied during the investigation. During the night of the murder she met up with Patrick
- who all were accused of the crime?
- How was the case eventually overturned for Amanda?
- The DNA evidence and fingerprints did not match up
- Who was eventually convicted for the murder
- Rudy Guede-he lived downstairs- which is where parties happened all the time
- Where was Amanda when she was finally cleared of the murder and why was she there?
- She was in Seattle because that is where she grew up.
- Today she is an advocate for those who have been wrongly accused and Jason Hawking?
Chapter 4: death
- Role of forensic pathologist: Someone who studies death and disease- determines cause of death
- Coroner is an elected official
- history of coroner-
- may not have medical degree
- Medical examiner is appointed and usually physician who is board certified
- history of coroner stems from king richard I for the purpose of collecting money and personal possessions from deceased families (death tax)
- what a pathologist might do at the scene of the crime
- protect scene and body
- removal of body in medically acceptable manner
- critical phase of death investigation will be preliminary reconstruction of events that preceded the onset of death, all significant details of scene is recorded
- blood splatter and blood flow prints are documented
- tire marks or shoe prints must be documented
- fingerprints must be processed and collected
- evidence dropped or discarded by perpetrator is collected
- weapons, fired bullets, and casings are collected
- photographs taken before scene is altered in any way
- critical phase of death investigation will be preliminary reconstruction of events that preceded the onset of death
- The cause and manner of death is done through autopsy which can only be performed by forensic pathologist
- cause of death
- primary objective of autopsy is to determine cause of death
- some common causes of death: blunt force injury, sharp force injury, sharp force injury, asphyxia, gunshot wound, and substance abuse
- blunt force injury
- cause by non sharpened object
- can abrade tissue or cause contusion- arising from bleeding from tiny ruptured blood vessels
- being able to determine age of bruise is very problematic and unreliable
- sometimes contusion can take the shape of the offending object
- Sharp force injury
- come from weapons with sharp blades
- a cut is longer than it is wide
- a stab is deeper than it is long
- these types of scenes are very bloody, blood may be found at different locations throughout the crime scene
- important to look for defence wounds (forearms or legs) if there are none, victims may have been unconscious or tied up during assault.
- Asphyxia
- interference with intake of oxygen- toxic gas or carbon monoxide
- death at fire is caused primarily by toxic gas carbon monoxide
- carbon monoxide binds to red blood cells and will be present after death which will indicate if victim was breathing at the time of the fire
- Hanging:
- victims die from loss of blood flow to/from the head and may show petechiae-blood vessels burst inside of eyelid
- important to note exactly how victim was found, position of nose, and type of knot used
- SMothering:
- blocking mouth or nose with some object
- these types of crimes are homicidal and accidental
- typically happens in infants or where victim is trapped under something
- gunshot wound
- the distance weapon was fired from a target is one of most important factors in characterising gunshot wound
- if firearm was fired from several feet away, it rules out suicidee
- autopsy must include determination of pagth/”wound path of projectile
- pathologist will collect all projectiles from body
- gunshot wound may not explain how victim died’ it can take several minutes or hours for person to bleed out or infection may be ultimate cause
- substance abuse
- toxicological tests are performed every autopsy because it’s so common
- large problem in us
- not all illegal drugs lead to death
- many drugs can be detected at very low levels
- drugs can cause death or be contributing factor to one’s death
- also source of acts of violence that lead to death
Coroner history:
The coroner originated in England during the 11th century. Originally, they were labelled “crowner” or “coronator. The word coroner derives from the Latin root word coroner, which is Latin for officer of the crown. The coroner system was first created when Richard I was in power. The king and the sheriff were concerned that they were not receiving all of the death tax money. King Richard thus appointed a coroner to keep track of all the deceased and make sure their taxes were paid. During 1194, Richard the Lionhearted officiated the coroner system.
Works cited
Britannica, The Editors of Encyclopaedia. “coroner”. Encyclopedia Britannica, 27 Sep. 2007, https://www.britannica.com/topic/coroner. Accessed 13 September 2022.
- The autopsy- what is it and why is it done?
- autopsy in broadest sense
- The examination of the body after death
- Goal of autopsy is to determine cause of death and manner of death
- Cause of death determination includes the autopsy, history, history of death, witness statements, relevant medical records, and any crime scene investigation
- what kind of evidence can be recovered from autopsy
- Medical examiner carefully examines the victim to establish cause and manner of death
- Tissues and organs will be retained for pathological (disease) and toxicological examination
- Arrangements made between examination and investigator to secure a variety of items that may be obtainable from the body for laboratory examination
- THe following can be collected and sent to forensic laboratory
- Victims clothing
- Fingernail scrapings
- Combings from head and pubic hairs
- Buccal swab
- Vaginal, anal, and oral swabs
- Recovered bullets from body
- Hand swabs form shooting victims
- Swabs of body areas suspected of being in contact with dna
- Hand stubs or swabs for gunshot residue analysis
- What types of examinations are done during an autopsy and why?
- external examination:
- Broad overview of condition of body and clothing
- Consists of classifying injuries-distinguishing between different types of wounds
- internal examination:
- Dissection of human body
- Remove all internal organs through y shaped incision beginning at top of each shoulder and extending to pubic bone
- weighing , dissecting, and sectioning each organ of the body
- Toxicology
- Internal examination is where toxicological specimens are taken
- Samples of blood,stomach content, bile, and urine
- Blood is tested to determine the presence and levels of alcohol and drugs
- Some drugs redistribute the blood after death and thus complicate the interpretation of postmortem blood levels of these drugs
- manner of death
- what is the difference between cause of death and manner of death
- Manner of death: relates to the circumstances that led to the fatal result
- what are the 5 manners of death
- Homicide
- Non accidental death caused by grossly, negligent, reckless, or intentional actions of another person
- Suicide
- Result of person taking their own life
- Must be determined that the individual carried out the act alone
- Victims personal history, psychiatric history, past attempts, past threats, or even a note will be taken into account
- Accidental
- There must not be an intent to cause harm on part of the perpetrator or victim
- All cases must have toxicology report carried out
- Autopsy should be able to correlate what the crime acne indicates
- Determination of manner of death in drowning, falls, and asphyxiation can be extremely difficult
- natural causes
- Distinction between natural death and accidental is challenging
- Classification of natural death includes disease, chronic drug abuse, alcohol abuse, longtime exposure to natural toxins or asbestos
- Drug abuse has to be proven that a lethal amount was purposefully taken in order for it to be a suicide
- Undetermined
- When rational classification cannot be determined
- Can happen when the mechanism that caused the death cannot be determined by a physical finding in the autopsy-or lack of meaningful findings in the subsequent toxicology report.
- Homicide
- what is the difference between cause of death and manner of death
- estimating time of death:
- Pathologists can never give exact time of death- some factors can be analysed to determine approximate time of death
- Witness statements help with events leading up and after the crime, physical changes must be accounted for as well,
-
- what is algor mortis, livor mortis, and rigor mortis?
- algor mortis:
- Results in the loss of heat by a body
- Body loses heat by 1 to 1 ½ degrees fahrenheit per hour until body reaches environment temp- can be used to determine time of death
- Factors that change this are ambient temp, body size, and victims clothing
- livor mortis:
- Results in settling of blood in areas of body closest to ground- occurs from 20 minutes to three hours of death ad continues up to 16 hours, after all point of lividity/colour is fixed
- Lividity can be blanched (turn white) when pressed until it becomes fixed. Good indicator that body was moved after death
- rigour mortis:
- Results in shortening of muscle tissue and stiffening of body parts in the position at death (occurs within first 24 hours and disappears within 36 hours)
- Conditions that are hot can speed up this condition along with physical activity that occurred prior to death
- Individuals with limited muscle mass (infants, elderly, obese) may not develop rigour completely.
- algor mortis:
- what does potassium levels in the eyevak fluids tell pathologists
- potassium eye levels: Forensic pathologists can determine amount of potassium in vitreous humour of eyeball and use it to approximate time of death
- The time at which potassium is released can be used to approximate the time of death
- What does the contents of the stomach tell a pathologist? Why is it important?
- stomach contents: may help determine the location of the victim prior to death
- May also have undissolved pills
- What does decomposition tell a pathologist?
- decomposition: once decomposition has set in, previous methods are not usable. After death two things take place: Autolysis and putrefaction
- what is algor mortis, livor mortis, and rigor mortis?
- Autolysis
- Self regulation of cell destruction by enzymes
- Dependent on mechanism of death
- putrefaction
- Decomposition carried out by microorganisms such as bacteria
- Accompanied by bloating, discoloration, foul smell caused by accumulating gases
- Dependent on mechanism of death
- Role of forensics anthropologist:
- Forensic anthropology is concerned with the identification and examination of human skeletal remains
- Examination of bones can reveal sex, age, race, and skeletal injuries
- Gender can be determined by the size of pelvis and skull
- Female pelvis is wider and more circular
- recovering and processing remains
- Sites where human remains are found-treated a crime scene
- Scene must be secured and searched
- All aspects of evidence must be documented and collected
- Aerial photograph, metal detectors, ground penetrating radar, infrared photography, cadaver dogs
- Every bone needs to be photographed, tagged, and sketched and documented in notes
- Height of when victim was alive can be estimated by measuring long bones of skeleton
- Anthropologist may help create facial reconstructions to identify skeletal remains
- How can facial recognition help forensic anthropologists?
- Identify skeletal remains
- Only dna is required for comparison
- Can determine age, sex, and race
- Determine victim characteristics
- What can a skeleton tell an anthropologist about the identity of the victim?
- Are the bones human?
- how many individuals are represented
- How long ago did the death occur?
- What was the victim’s age?
- What was the person’s sex?
- What was the person’s ancestry?
- What was the person’s height?
- Are there any identifying characteristics such as old injuries, disease, or unusual features?
- What was the cause of death?
- What was the manner of death? (homicide, suicide, accidental, natural, or unkown)
- What different characteristics of the skeletal bones help to identify it as human, age of skeleton, male/female, type of death?
- What can a skeleton tell an anthropologist about the identity of the victim?
|
Female |
Male |
|
|
Cranium |
medium to large in size |
large in size |
|
forehead |
high in height, vaulted, rounded |
low in height, sloped, backward |
|
brow bone |
diminished |
pronounced |
|
mastoid process |
diminished or absent |
pronounced |
|
mandible angle |
obtuse (>90 deg) |
approx. right angle (90 deg) |
|
pelvis opening |
wide, circular |
narrow, non circular, heart shaped |
|
sacrum |
short, wide, turned outward |
approx. equal width/length, turned inward |
|
subpublic angle |
approx. right (90 deg) |
acute (<90) |
|
femur |
narrow, angled inward from pelvis |
thick, relatively straight from pelvic |
|
overall skeleton |
slender |
robust |
|
age (months) |
closure |
|
6-9 |
mandible fused |
|
4-6 |
humorous head bone fuse |
|
7-8 |
pelvis frontal bones fuse |
|
4-16 |
femur shaft sections built |
|
9-13 |
elbow bones fuse |
|
10 |
finger bones fuse |
|
16-18 |
femur head fused to shaft |
|
18 |
Wrist bones fused |
|
18-21 |
Humeral head bone fused to shaft |
|
18-24 |
Sternum fused to clavicle |
|
20-25 |
Pelvic bones fully formed |
|
21-22 |
Clavicle fused |
|
21-30 |
Lambdoidal suture fused (back of head) |
|
24-30 |
Sacrum bones fused |
|
30-32 |
Sagittal suture (centre of cranium) fused |
|
48-50 |
Coronal suture (front of cranium) fused |
|
Eye orbitals |
Nasal cavity |
incisors |
Cranium frontal plane |
|
|
Caucasoid |
oval |
Long, narrow |
smooth |
flat |
|
mongoloid |
circular |
small , rounded |
Shovelled interior |
Flat or projected outward |
|
negroid |
square |
wie |
smooth |
Projected outward |
|
Caucasoid (all measurements in centimetres) |
Negroid (all measurements in cm) |
Unknown (all measurements in cm) |
|
|
Female |
Height = femur length x 2.47+54.10 |
height=femur length x 2.28 + 59.76 |
Height= femur length x 3.01 + 32.52 |
|
Height = humerus length x 3.36 + 65.53 |
height= humorous length x 3.08 + 64.67 |
Height= humorous height x 4.62 + 19.00 |
|
|
Male |
height= femur x 2.32 + 65.53 |
Height = femur x 2.10 + 72.22 |
height= femur x 2.71 + 45.86 |
|
height= humorous x 2.89 + 78.10 |
height= humerus length x 2.88 + 75.48 |
height= humerus x 4.62 + 19.00 |
- Role of forensic entomologist- study of insects and their relation to criminal investigation, used to estimate time of death
- By determining the oldest stage of fly found on body, entomologist can approximate the postmortem interval
- Most common and important is the blowfly- green or blue
- After decomposition necrophagous insects descend on body within 24 hours depending on environment
- Typically a blow fly can lay 2,000 eggs-laid in human remains and hatch into maggots which eat human tissues and organs
- Can determine approximate time for how long a body has been left exposed by examining stages of fly larvae
- Time required for fly stage development is affected by location, climate, weather, and drugs
- Some beetles show up to eat blowflies, as body dries omnivorous insects feed on body and nearby vegetation (ants and wasps) spiders are the last to show up
- Other contributions
- Evidence should be collected by forensic entomology expert who takes temperature readings and take specimens from every area
- First arriving insects like moist warm open wounds, most insects found on body will likely ingest any drugs in the system and be good evidence
- If insects found extensively on hands and arms indicates defensive wounds
- George zimmerman case: self defence or murder
- Questions:
- Who was trayvon martin
- Trayvon martin was a 17 year old boy who was victim to a murder
- Who is george zimmerman
- George Zimmerman was a 38 year old neighbourhood patrol who killed Trayvon
- What are the details of that night that ended with the death of trayvon
- Zimmermann was on his way to the target when he noticed Travon walking slowly down the street looking at houses. George called the police to report him while following him. Trayvon thought he was being creepily followed, so he began to run to “escape”.The police dispatcher told Zimmerman to meet a policeman by the mailboxes. When he arrived, he got out of his car and Travon was hiding between bushes and attacked him. . Trayvon was pinned to the ground and he reached up to shove Zimmermann off him. Zimmermann was armed, so he believed that the boy was trying to grab the gun to shoot him. Zimmermann shoved Trayvon to the ground, grabbed his gun, and shot him.
- Why did the police dispatcher tell zimmerman not to follow trayvon and why was that not considered an order
- The police told Zimmerman to not follow him because he would be giving chase. It was not considered an order because it was more of a suggestion, they said they didn’t need him to do that, instead of do not do that.
- What evidence leads one to believe zimmerman’s side of the story and not that that the events of that night happened the other way around with words on top of trayvon
- The distance of the gun is consistent in zimmerman’s story
- Photos of zimmerman’s wounds prove he was trying to defend himself
- Gunpowder was found on trayvon’s chest, supporting zimmerman’s claim that they were on top of each other when he killed the boy
- Was it wise for the prosecution to not make this about race? Why do you think so?
- I think it was wise, Zimmerman’s self defence was not racially motivated. The case had very little to do with race.
- Why did Rachel Jentel not end up being the state’s war witness? What about her testimony made jurors feel uncomfortable and not help endure her to their hearts
- She had difficulty with literacy and speaking. SHe also lied to the court claiming that she didn’t want to talk about Trayvon because she was the last person he talked to.
- Who gave the defence their best testimony and what was it about
- Jon Good gave the best testimony because he saw the fight. He testified that Martin was on top of zimmermann and that zimmerman was the one calling for help.
- What evidence does lisa bloom give for the prosecution’s half hearted attempt and inability to win what she thinks is a winnable cas
- She says that Trayvon could not have seen the gun and would have needed X-ray vision. In several accounts, Zimmerman’s shirt came up and it was very easy to see the gun behind his back. She also claimed that this “murder” was racially motivated.
- Who was trayvon martin
- Harold shipman, aka Dr. Death
- What kind of doctor was Shipman
- He was a general practitioner
- Did Dr shipman ever lose his licence to practise medicine prior to being arrested
- What was the name of the last victim that ended up being his demise
- Kathleen Grundy
- How did dr shipman finally get caught
- Morphine was found in Kathleen’s body
- What year is most likely the first time that he killed his patients
- 1975
- What are the 3 predominating theories about why Dr Shipman killed his patients?
- Seeking to avenge the death of his mother
- He was addicted to killing
- He was practising euthenasia
- How did Dr shipman avoid being caught sooner than the death of kathleen grundy
- He would kill old women who were most likely going to die soon, and blame it on old age
- How many and what convictions did shipman receive
- He was convicted of murdering 15 of patients under his care
- What evidence was presented at his trial by the prosecution
- Kathleen’s will was forged with no acknowledgment to her children
- Kathleen’s body was exhumed and morphine was found in her body
- Shipman was the last person to see her alive
- where is dr shipman today
- He commited suicide in August, 2022
- What kind of doctor was Shipman
Ch 5: properties of matter and glass
- Physical vs chemical properties
- Physical properties such as weight, volume, colour, boiling paint, and melting point describe a substance without any reference to any other substance
- Chemical property:
- Describes the behaviour of a substance when it reacts or combines with another substance
- Measurement system
- Scientists used metric system except usa
- Metric- metre, gram, and litre
- 1 inch- 2.54 cm
- 1 lb-453.6 g
- 1 L- 1,06 qt
- Deci = 1/10 or 0.1
- Centi = 1/100 or 0.01
- mili = 1/100 or 0.001
- Micro = 1/1000000 or 0.000001
- Nano = 1/1000000000 or 0.000000001
- Kilo = 1,000
- Mega = 1,000,000
- Nature of matter
- element= simplest substance known and provides the building blok rom which all matter is composed
- All elements are in periodic table
- Two or more elements form a compound
- Atom is basic particle of an element and matter is smallest unit of compound
- Matter is anything that has a mass and occupies space
- element= simplest substance known and provides the building blok rom which all matter is composed
- States of matter
- Solid
- Liquid
- gas/vapour
- Substances can change from one phase to another without forming a new chemical species
- Sublimation- substance goes from solid straight to gas (dry ice)
- Theory of light
- Two models describe behaviour of light
- Light is described as wave of light, continuous wave
- Stream of discrete energy particles
- When white light passes through prism, it is dispersed into continuous spectrum of colours
- Visible light ranges in colour from red to violet in electromagnetic spectrum
- Wavelength: ds=istance bet two successive crests (or troughs) measured in nanometre
- Frequency: number of crests (or troughs) passing any one given point per unit of time- cycles per second cps
- Frequency and wavelength are inversely proportional to each other F=C/wavelength
- Electromagnetic spectrum is entire range of radiation energy from most energetic cosmic rays to least energetic radio waves
- Visible light is only small part of electromagnetic spectrum
- Wavelength and frequency are inversely proportional
- Two models describe behaviour of light
- Light as a wave
- Dispersion: process of separating light into its component colours when you pass light through prism it slows the different wavelengths down causing it to exit the prism as respective colours
- Refraction” bending of light waves because of a change in velocity
- Such as when light passes through glass
- Electromagnetic radiation moves through space, its behaviour can be described as continuous wave
- Once radiation absorbed by substance, discrete particles of light known as photons
- Helps forensic scientists as they determine what substance is by the type and quantity of light it absorbs
- Important physical properties
- Temperature is a measure of heat intensity
- Most commonly used temperature is celsius scale
- Celsius is derived by assigning freezing point of water at 0 and its boiling at 100
- Temperature is a measure of heat intensity
- Temperature scale
- Farhenheit is based of freezing point of 32 and boiling point is 212, difference between two is 180 units
- Celsius is 1/100 of the temperature change between the two points
- Weight: the force with which gravity attracts a body
- W=mg (g=gravity)
- Mass: amount of matter an object contains independent of gravity- when you go to the moon your weight changes but your mass is the same
- Mass of an object is determined by comparison to the known mass of standard objects
- Density defined as the mass per unit volume: D=M/V
- Density is intensive property of matter- remains same regardless of sample size
- Density considered a property of a substance and can be used as aid in identification
- volume can be calculated by placing it on cylinder of a known body of water (V1) and then measuring the new volume (V2) and then subtracting the difference (V2-V1) to fund density
- Light waves travel in air at constant velocity until they penetrate another medium at which point they are suddenly slowed,d causing waves to bend
- Bending of light waves bc of a change in velocity- refraction
- Refractive index is the ratio of the velocity of a light in a vacuum to that in the medium under examination
- 25 celsius the refractive index of water is 1.333- means that light travel 1.333 times faster in vacuum than water
- Refractive index is an intensive property and will serve to characterise a substance
- Crystalline solids have definite geometric forms bc of the orderly arrangement of atoms
- These solids refract beam of light in two different light ray components
- Results in double refraction
- Birefringence is numerical difference between two refractive indices
- Not all solids are crystalline in nature- glass has random arrangement to dorm amorphous (non crystalline) solid
- Glass fragment
- Glass is hard, briuttle, amorphous substance composed of silicon oxides mixed with various metal oxides
- Amorphous solids have their atoms arranged randomly unlike crystals
- Tempered glass is stronger than normal glass due to rapid heating and cooling
- Laminated glass is found in car windshield has a layer of plastic between two pieces of window glass
- For forensic scientist problem of glass comparison is one that depends on the need to find and measure those properties that will associate one glass fragment with another while minimising or eliminating other sources
- To compare glass fragments a forensic scientist evaluates two important physical properties: density and refractive index
- Floatation method
- Precise and rapid method for comparing glass density
- A glass particle is immersed in liquids
- Density of liquid is carefully adjusted by the addition of small amounts of appropriate liquid until glass chip remains suspended in liquid
- At this point glass will have same density as liquid and can be compared to relative pieces of glass
- Immersion method
- Flotation and immersion are best used to determine a glass fragments density and refractive index
- Latter involves immersing a glass particle in a liquid medium whose refractive index is varied until it is equal to that of a glass particle
- At this point, known as match point, the becke line disappears and minimum contrast between liquid and particle is observed
- Becke line is a bright halo near the border of a particle that is immersed in a liquid of a different refractive index
- Analysing cracks
- [penetration of window glass by projectile produces cracks which radiate outward (radial fractures) and encircle the hole (concentric factore)
- By analysing the radial concentric fracture patterns in glass the forensic scientist can determine direction of impact
- High velocity projectile such as bullet leaves hole that is wider at exit side and hence its examination is important when determining the direction of impact
- Direction of impact can be accomplished by applying 3R rule: radial cracks form right angle on the reverse side of force
- Sequence Of impacts when there have been successive penetrations of glass is frequently possible to determine because the fracture always terminated art an existing line fracture
- Collection of glass
- In the remotest possibility exists that glass fragments may be pierced together, every effort must be made to collect all glass found
- When an individual fit is though improbably, the evidence collector must submit all glass evidence found in the possession of the suspect along with a representative sample of broken glass remaining at the crime scene
- glass fragments should be packaged in solid containers to prevent further damage
- Isf suspects shoes or clothing are examined for glass fragments, they should be individually wrapped in paper and transmitted to laboratory
- Charles Lindbergh baby kidnapping:
- https://www.fbi.gov/history/famous-cases/lindbergh-kidnapping
- Who was charles?
- Who were the suspects
- Who all “helped” in trying to recover the child
- What ransom was paid
- How was the suspect caught
- What evidence was used to convict
Ch 5.5: Polarising microscopy:
- Determination of these refractive index data provides info that helps to identify minerals present in a soil sample or identity of a man made fibre
- The microspectrophotometer is spectrophotometer coupled with a light microscope
- The examiner studying a specimen under a microspectrophotometer can simultaneously obtain the visible absorption spectrum or IR spectrum of the material being observed
- The spectrophotometer is not well suited for examining small particles found as evidence
- The microspectrophotometer is useful in the examination of trace evidence, paint, fibre, and ink evidence
- The scanning electron microscope SEM bombards a specimen with a beam of electrons instead of light to produce a highly magnified image from 100x to 100,000x
- The depth of focus of the SEM is some 3oo times better than optical systems at similar magnification
- The bombardment of the specimens surface with electrons normally produces x ray emissions that can be used to characterise elements present in material under observation
- Of many plant species on earth more than half a million produce pollen or spores
- Each species has a unique pollen grain or spore which can be helpful in criminal cases
- Person who is trained in studying pollen and spores from crime scene if called a forensic palynologist
- Microscopy is the main tool in forensic palynology
- Pollen grains are the single celled male gametophytes in seed bearing plants
- Spores consist of both male and female gametes of plants such as algae, fungi, mosses. And ferns
- It may be possible to identify a geographical origin of the pollen samples retrieved from suspects clothing simply by the type and percentages of airborne pollen grains
- Other plants produce sticky pollen grains thus it is rare to find it on clothing unless direct contact was made with the plant by the suspect
- Spores and pollen are microscopic and reproduce in the millions
- Compound light microscope with magnification up to 1000 can be used to look at grains and spores
- SEM is most productive
- Unique shapes and surface ornamentation are typically used to identify spore samples- pollen uses surface ornamentation and external wall sculpting for identification
- To avoid destruction or contamination, early collection of samples for analysis is important
Ch. 6: Drugs
- French connection:
- It was one of the most famous global drug smuggling and selling operations in History-2 movies made from it
- Involved gangs in Corsica, italy, organised crime in US, farmers in turkey (it was legal to grow poppies) and NYC police
- Was a scheme that involved growing opium in Turkey, processing heroin in France-smuggling it into US
- At time it provided nearly all the white heroin in US
- After WWII shipments and seizures of shipments picked up, one occasion US seized 45 LBS
- By 1960 5000 LBS-coming into us from france
- 1971-us and france were able to get turkey to outlaw poppy growing- arrests made in turkey and france
- After heroin started coming from south and central america where it was more brown and became known as mexican mud
- Introduction
- Drug can be defined as natural or synthetic substance used to produce physiological or psychological effects in humans or other higher order animals
- Narcotic drugs are analgesics-they relieve pain by depressing action on central nervous system
- This affects blood pressure, pulse rate, and breathing rate
- What is an Illicit drug?
- Most drugs are legitimate and prescribed for illnesses, injuries or other medical purposes
- Drug abuse is when people take drugs for other purposes other than intended use
- F
- Controlled substances are those drugs that are listed in federal code “Controlled Substance Act”
- Regular use of a narcotic drug invariably lead to physical dependence
- Most common source for these narcotics is opium-extracted from poppies grown in turkey and afghanistan
- Drug dependence
- Drug dependence is chronic, progressive disease characterised by significant impairment that is directly associated with persistent and excessive use of a psychoactive substance
- Drug abuse has grown from problem with members of lower social class to a problem with every class
- 23 million americans affected today
- More than 75% evidence evaluated in crime labs is drug related
- Drug Dependence
- Some of first drugs to be regulated by law enforcement were “habit-forming” drugs- cocaine and marijuana
- Cocaine was first banned in early 1900s as wealthy americans didn’t like effect
- They saw it having on “lower class” citizens
- US has been trying to control illicit drugs for nearly century
- The “war on drugs” has been a failure
- Dependence on drugs can exist in numerous patterns in all degrees of intensity
- Depends on: nature of drug, route of administration. Dose, frequency of administration, and individuals metabolism
- Nature and significance of drug dependence must be considered when deciding a drugs danger to society
- Viewed from two aspects of human behaviour-physiological dependence and physical dependence
- Psychological dependence
- Is conditioned use of drug caused by emotional need- you would rather have drugs than eat
- This varies widely with drug and the individual-not all drug users are addicts
- Drugs like alcohol, heroin, amphetamines, barbiturates, and cocaine cause high degree of psychological dependence
- Other drugs like cocaine and marijuana have low degree of dependence, although high use can cause high degree of dependence
- 46 states have legalised marijuan in some form the federal government has not moved it off their list of highly abused drugs
- The federal government does not recognize any medical benefit to marijuana
- Physical dependance
- Physical dependence is physiological need for drug characterised with withdrawal sickness when administration of drug is abruptly stopped
- Once drug user stops a drug routine, sever physical sickness can follow such as chills, vomiting, stomach cramps, convulsions, insomnia, pain, and hallucinations
- Drugs like marijuana, LSD, and cocaine creates strong anxieties when repeated use is discontinued
- Societal aspects of drug use
- Social impact of drug dependence is directly related to extent to which user has become preoccupied with drug
- More frequently the drug satisfies the person’s needs, the greater the dependence
- Extreme drug dependence may lead to behaviour that has serious implications for public’s safety, health, and welfare
- Many forms of drug dependence do not carry sufficient adverse social consequences to warrant the prohibition such as tobacco and coffee
- Disastrous failure of banning alcohol in US in 1920s (prohibition)
- Opiates
- Used to treat pain or cause sleep-made from poppyseed
- Morphine is readily extracted from opium-used in medicine to block pain receptors during surgery-used to synthesise heroin
- Addicts frequently dissolve heroin in water by heating it in spoon and injecting it in skin
- Heroin produces high that is accompanied by drowsiness and sense of well being that lasts three hour
- Codeine is also present in opium, but it’s usually prepared synthetically from morphine
- oxycontin -active ingredient is oxycodone-not derived from opium or morphine but has same physiological effects on body as do opium narcotics
- Other opiates
- Oxycontin is prescribed to a million patients for treatment of chronic pain
- Methadone is another well known synthetic opiate
- Methadone is pharmacologically related to heroin, it appears to eliminate addict’s desire for heroin while producing minimal side effects
- Hallucinogens
- Another class of drugs is hallucinogens
- Marijuana is most well known member of this class
- Cause marked changes in normal thought processes, perceptions and moods
- Most controversial drug in this class- its long term effects on health are still largely unknown
- Health benefits are to reduce hyper anxiety in people and also reduce seizures in people with epilepsy
- Another class of drugs is hallucinogens
- Marijuana
- Refers to preparation derived from plant cannabis
- Chemical substance largely responsible for the hallucinogenic properties of marijuana is known as tetrahydrocannabinol, or THC
- THC content of cannabis varies in different parts of plant, generally decreasing in the following sequence: Resin, flowers, and leaves with little THC in stem, roots, or seeds
- THC rich resin is known as handish
- Marijuana does not cause physical dependence but risk of harm is in heavy long term use
- Some effects of long term use are: increased heart rate, dry mouth, reddened eyes, impaired motor skills and concentration, increased hunger and increased desire for sweets
- Other hallucinogens
- Include LSD, mescaline (Peyote), PCP. psilocybin and MDMA (Ecstasy)
- LSD is synthesised from lysergic acid, can cause hallucinations that last 12 hours
- Can occur a few years after stopping use of LSD
- PCP often synthesised in clandestine laboratories and is smoked, ingested, or sniffed
- Phencyclidine is often mixed with other drugs, such as LSD or amphetamines, and is sold ad power (called angel dust) capsule or tablet
- Oral intake of PCP first leads to feelings of strength and invulnerability, may turn to depression, tendencies towards violence, and suicide, along with dreamy sense of detachments
- Paranoia accompanies its use along with sudden schizophrenia behaviours days later in some users
- Depressants
- Another class of drugs
- Depressants are substances used to depress functions of central nervous system
- Depressants calm irritability and anxiety and may induce sleep
- Includes alcohol, ethanol (only alcohol that is safe for human consumption), bertiuaries, tranquilisers, and sniffing substances, aeroplane glue, model cement, or aerosol gas propellants such as Freon
- Alcohol
- Alcohol enters body’s bloodstream and travels to brain where it acts to suppress the brains control of thought process and muscle coordination
- Behavioural patterns of alcohol intoxication vary depending on social setting, amount consumed, and personal expectation to individual
- Effects start with first drink and include slowing of judgement, memory, concentration, reduces coordination, inhibits orderly thought and speech, and slow reaction time
- Depressants
- Barbiturates or downers are taken orally and create a feeling of well being, relax body, and produce sleep
- Tranquilisers, like antipsychotics and antianxiety, unlike barbiturates, produce a relaxing tranquillity without impairment of high thinking faculties or inducing sleep
- Sniffing (Huffing) has immediate effects, such as exhilarations but it impairs judgement and may cause liver, heart, and brain damage or sometimes death
- Stimulants
- Classification of stimulants includes amphetamines, known as uppers or speeds, and cocaine, also called crack
- Stimulants are substances taken to increase alertness or activity, followed by decrease in fatigue and loss of appetite
- Amphetamine and methamphetamine injected intravenously-cause initial rush by intense pleasure
- This is followed by period of exhaustion and prolonged period of depression
- Cocaine “discovered” by sigmund freud about 1884 extracted from leaves of erythroxylum coca causes increased alertness and vigour accompanied by suppression of hunger fatigue and boredom
- Crack is cocaine mixed with baking soda and water then heated
- Crack is often smoked in glass pipes like cocaine-stimulates brain’s pleasure centre
- Heavy use causes paranoia, voices in one’s head, and suspicion that they are being watched, few cracks users get cured
- Club drugs
- Refers to synthetic drugs that are used at nightclubs, bars, and raves
- Substances that are often used as club drugs include MDMA (ecstasy) GHB (gamma hydroxybutyrate) Rohypnol (“Roofies”) ketamines and Methamphetamine
- GHB aand rohypnol are central nervous system depressantrs that are connected with drug facillitated sexual assault, rape, and robbery
- methylenedioxymethamphetamine , also called MDMA, or ecstasy is synthetic mind altering drug that exhibits many hallucinogenic and amphetamine like effects
- Ecstasy enhances self awareness and decreases inhibitions- however seizures, muscle breakdown, stokes, kidney failure, and cardiovascular system failure often accompany chronic abuse
- Ketamine is primarily used as a veterinary animal anaesthetic that in human causes euphoria and hallucinations
- Ketamine can cause impaired motor functions, high blood pressure, amnesia, and mild respiratory depression
- Anabolic steroids
- Another category of drugs
- These are synthetic compounds that are chemically related to male sec hormone testosterone
- anabolic steroids are often abused by individuals who are interested in accelerating muscle growth
- Side effects include unpredictable effects on mood and personality, depression, diminished sex drive, halting bone growth, and liver cancer
- Control of illicit drugs in US
- What determines if drug is prohibited or controlled
- Public interests, morality, personal choice, social order, and health use
- In 1906 first federal law was passed to help control some drugs
- Pure food and drugs act- first truth in labelling act
- Public reaction of chinese immigrants and their smoking of opium, and rise of cocaine use
- 1914-second federal law created
- Harrison act-registration of and a special tax on producing, importing, manufacturing, deal in, dispense, or give away opium
- 1930 congress formed Bureau of narcotics within treasury department
- Stepped up in law enforcement against narcotics, cocaine, and marijuana
- After WWII half of all crimes were drug related
- 1956 we get narcotic drug control act-increased penalties for illicit drug use
- Stiffer jail sentences
- Death penalty for selling to minor
- 1970-comprehensive controlled substances act
- Control of drugs became direct federal law enforcement activity
- Enforcement was moved to justice department (creation of DEA)
- Decision on which drugs are controlled rets with Sec of health and human services who delegates to the FDA
- Under this law tobacco and alcohol products are excluded
- What determines if drug is prohibited or controlled
- Drug control laws
- us federal law known as controlled substance act will serve to illustrate a legal drug classification system created to prevent and control drug abuse
- This federal law establishes five schedules of classification for controlled dangerous substances on the basis of a drugs:
- Potential for abuse
- Potential for physical and psychological dependence
- Medical value
- Schedules of classification:
- Schedule I drugs such as heroin, marijuana, methaqualone, and lsd have high potential or abuse and currently have no accepted medical use
- Schedule II drugs like cocaine, PCP, and most amphetamine and barbiturate prescriptions have high potential for abuse but have medical uses within severe restrictions
- Schedule II drugs like codeine and anabolic steroids have less potential for abuse and have currently accepted medical use such as all barbiturate prescriptions not covered under schedule II
- Schedule IV drugs such as darvon, phenobarbital, and some tranquillisers, such as diazepam (valium) and chlordiazepoxide (librium) have low potential for abuse and have current medical use
- Schedule V drugs such as opiate drug mixtures that contain non-narcotic medicinal ingredients must show low abuse potential and have medicinal use (ibuprofen)
- Criminal Penalties under the act
- Criminal penalties for unauthorised manufacture, sale, or possession of controlled substances are related to schedule
- Schedule I and II for a first offence carry up to 20 years in prison and or a 1 million dollar fine for an individual or 5 million for other than an individual
- Synthetic drugs are also placed in these categories as they are discovered-such as fentanyl (designer drugs)
- The controlled substance act also takes into account the clandestine drug labs
- Drug identification
- Challenge or difficulty of forensic drug identification comes in selecting analytical procedures ty will ensure a specific identification of drug
- This plan is divided into two phases
- Screening test: is nonspecific and preliminary in nature to reduce the possibilities to a manageable number
- Confirmation test: single test that specifically identifies a substance
- Conformational determinations
- Once this preliminary analysis is completed, conformational determinations is pursued
- Typically infrared spectrophotometry or gas chromatography mass spectrometry is used to identify drug substance specifically
- Colour tests:
- Many drugs yield characteristic colours when brought into contact with specific chemical reagents
- This can be used not only in lab bt field as well
- This is a screening test and not a conclusive identification test
- Marquis reagent turns purple in the presence of heroin
- Dilli koppANYI turns violet blue with barbiturates
- Duquenois- levine turns purple with marijuana
- Van Urk turn purple blue with LSD
- Scott test turn cobalt thiocyanate Solution, blue with cocaine
- Microcrystalline tests
- Technique considerably more specific than colour test
- A small drop of chemical reagent is added to small portion of drug
- After short time crystals form, size and shape are unique to each drug
- These tests are rapid and often do not require isolation of drug from its diluents
- Qualitative vs quantitative
- Another consideration in selecting an analytical technique is need for either quantitative or qualitative determination
- Qualitative relates to just the identity of material, quantitative requires determination of percent of components of a mixture
- Chromatography
- Means of separating and tentatively identifying components of mixture
- Theory of chromatography is based on observation that chemical substances have tendency to partially escape into surrounding environment when dissolved in liquid or when absorbed on a solid surface
- Those materials that have preference for the moving phase will slowly pull ahead and separate from those substances that prefer to remain in stationary phase
- TLC
- The liquid will slowly rise up the plate by capillary action causing the sample to become distributed between stationary phases and moving to liquid phase
- Because most compound are colourless, materials must be visualised by placing plates under ultraviolet light or spraying plate with chemical reagent
- Distance a spot travels up thin layer plate can be assigned numerical value, known as Rf value
- Gas chromatography
- iGC, moving phase is a gas called carrier gas, which flows through a column
- Stationary phase is thin thin of liquid contained within column
- After mixture has traversed length of column, it will emerge separated into its components
- The written record of this separation is called chromatogram
- Time required for component to emerge from CG column is known as retention time
- Spectrophotometry
- Just like substance can absorb visible light to produce colour, many of invisible radiations of electromagnetic spectrum are likewise absorbed
- Spectrophotometry measures quantity of radiation that a particular material absorbs as function of wavelength and frequency
- Quantity of light absorbed at any frequency is directly proportional to concentration of absorbing species is known as Beer’s LAw
- UV and IR spectrophotometry
- Currently most forensic labs use UV and IR spectrophotometer to characterise chemical compounds
- Simplicity of UV spectrum, facilities it use as a tool for determining a materials probably identity, although it may not provide definitive result
- IR spectrum provides a far more complex pattern
- Different materials always have distinctly different infrared spectra
- Each IR spectrum is therefore equivalent to a fingerprint of that substance
- Spectrophotometer
- Instrument used to measure and record the absorption spectrum of chemical substance
- Components of spectrophotometer are
- Radiation source
- Monochromator or frequency selector
- Sample holder
- Detector to convert electromagnetic radiation into an electrical signal
- Recorder to produce record of signal
- Absorption spectra can be done in the visible ultraviolet (UV) or infrared regions (IR)
- Mass spectrophotometry
- In mass spectrometer, beam of high energy electrons collides with material producing positively charged ions
- These positive ions almost instantaneously decompose into numerous fragments which are separated according to masses
- Unique feature of mass spectrometry is thar under carefully controlled condition, no two substances produce dame fragmentation pattern
- CG and Mass
- Direct connection between GC column and mass spectrometer allows each component to flow into mass spectrometer as it emerges from the GC
- Separation of mixtures components is first accomplished by GC
- Fragmentation of each component by high energy electrons in the mass spectrophotometer will produce distinct pattern, like fingerprint of substance being examined
- Collection and preservation:
- Field investigator is responsible ensuring that evidence is properly packaged and labelled from lab
- Common sense is the best guide, keeping in mind that package must prevent the loss of the contents and or cross contamination
- Often the original container in which drug was seized will suffice
- All packages must be marked with info that is sufficient to ensure identification buy the officer in future and establish chain of custody
- Thin layer chromatography
- TLC uses solid stationary phase coated onto a glass plate and mobile ;liquid phase to separate the components of mixture
- Preliminary Analysis
- Forced with the prospect that the unknown substance may be any one of a thousand or more commonly encountered drugs the analyst must employ a screening test to reduce possibilities to small number
- This objective is often accomplished by subjecting the material to a series of colour tests that will produce characteristic colours for commonly encountered illicit drugs
- Microcrystalline test can also be used to identify specific drug substances by studying the size and shape of crystals formed when drug is mixed with specific reagents
- COnformational determinations
- Once this preliminary analysis is completed, conformational determinations is pursued
- Typically infrared spectrophotometry or gas chromatography mass spectrometry is used to identify drug substance specifically
- Colour tests:
- Many drugs yield characteristic colours when brought into contact with specific chemical reagents
- This can be used not only in lab bt field as well
- This is a screening test and not a conclusive identification test
- Marquis reagent turns purple in the presence of heroin
- Dilli koppANYI turns violet blue with barbiturates
- Duquenois- levine turns purple with marijuana
- Van Urk turn purple blue with LSD
- Scott test turn cobalt thiocyanate Solution, blue with cocaine
Ch. 9: Forensic serology
- History of blood typing
- 1901-Karl landsteiner announced on of the most significant discoveries of the 20th century-typing of blood
- For years physicians had attempted to transfuse blood-typically with fatal results
- Karl Landsteiner’s work gave us a ABO system of blood typing
- This work opened up new fields of research in the biological sciences
- 1937-Rh factor in blood had been demonstrated
- Nature of blood
- The word blood refers to a highly complex mixture of cells, enzymes, proteins, and inorganic substances
- Plasma, which is the fluid portion of blood, is composed principally of water
- Red blood cells (erythrocytes), white blood cells (leukocytes), and are the platelets solid materials suspended in plasma
- Antigens, usually proteins, are located on surface of red blood cells and are responsible for blood type characteristics
- Blood typing
- More than 15 blood antigen systems have been identified but the ABO and Rh systems are the most important
- An individual that is type A has A antigens on his/her red blood cell, type B has B antigens , type AB has both A and B antigens, and type O has neither A nor B antigens
- Rh factor is determined by the presence of another antigen, the D antigen
- People having the D antigen are Rh ; those not having the D antigen are Rh negative
- For every antigen, there is specific antibody that will react with it to form clumps known as agglutination
- Thus if serum containing anti B is added to red blood cells carrying B antigen, they will immediately react
|
Blood type |
Antigen on RBCs |
Antibodies in serum |
|
A |
A |
anti-B |
|
B |
B |
anti-A |
|
AB |
AB |
Neither anti A nor B |
|
O |
none |
Both anti A |
- Serology
- The term serology is used to describe a broad scope of laboratory tests that use specific antigen and serum antibody reactions
- The identity of each of the four ABO blood groups can be established by testing the blood with anti A and anti B sera
- Serology antigen-antibody reaction
- The concept of specific antigen antibody reactions has been applied to immunoassay techniques for the detection of drug abuse in blood and urine
- Immunoassay
- A number of immunological assay techniques are commercially available for detecting drugs through antigen-antibody reaction
- One such technique, the enzyme multiplied immunoassay technique (EMIT) , is used by toxicologists because of its speed and high sensitivity for detecting drugs in urine
- In typical EMIT analysis antibodies that will bind to a specific drug are added to subjects urine
- Other immunoassay procedures are also available, such as radioimmunoassay (RIA), which uses drugs labelled with radioactive tags
- Emit
- A typical emit analysis begins by adding to a subjects urine, antibodies that bind to specific type or class of drug being looked for
- Common drug to be investigated is marijuana
- Active ingredient in marijuana is tetrahydrocannabinol (THC)
- The body converts THC to metabolites, such as THC-9 carboxylic acid, for easier excretion, which is easily detectable by EMIT
- Can be detected within 2-5 days of marijuana use, some individuals may be detected up to 30 days later
- Antigen-antibody reaction
- When an animal, such as a rabbit or mouse, is injected with an antigen, its body will produce a series of different antibodies, all of which are designed to attack one particular site on the antigen of
- This collection of antibodies is known as polyclonal antibodies
- Alternatively, a more uniform and specific collection of antibodies designed to combine with a single antigen site can be manufactured
- Such antibodies are known as monoclonals
- Polyclonal and monoclonal antibodies
- Polyclonal antibodies are antibodies that the body produces that are designed to attack multiple antigens
- Sometimes scientists need an antibody that us more specific and will attack only one type of antigen, called monoclonal antibodies
- These types of antibodies are being used in immunoassay test kits for abused drugs
- Monoclonal antibodies have also been approved by FDA for use in certain cancer treatments (lymphomas mostly)
- Forensics of blood
- The criminalist must be prepared to answer the following questions when examining dried blood
- 1.is it blood
- 2.from what species did the blood originate
- 3.if the blood is of human origin, how closely can it be associated with a particular individual
- The determination of blood is best made by means of a preliminary colour test
- The criminalist must be prepared to answer the following questions when examining dried blood
- Testing for blood
- A positive result from the Kastle Meyer colour test is highly indicative of blood
- Haemoglobin Causes a deep pink colour
- Alternatively, the Luminol test is used to search out trace amount of blood located at crime scenes
- A positive result from the Kastle Meyer colour test is highly indicative of blood
- Testing for blood
- Luminol produces light (luminescence) in a darkened area-can detect blood when diluted to as little as 1 in 100,000 ppm
- Microcrystalline test, Such as takayama and teichmann tests, depend on the addition of specific chemicals to the blood so that characteristic crystals will be formed
- Once the stain has been characterised as blood, the precipitin test will determine whether the stain is of human or animal origin
- The precipitin test uses antisera normally derived from rabbits that have been injected with the blood of a known animal to determine the species origin of a questioned bloodstain
- Once it has been determined that the bloodstain is of human origin, an effort must be made to associate or dissociate the stain with a particular individual
- Human bloodstains dried for 10-15 years or longer may still give a positive precipitation reaction. Even extracts of tissues from mummies 4-5 thousand years old have given positive reactions
- DNA analysis has allowed forensics scientists to associate blood with a single individual
- ABO vs DNA
- Prior to the advent of DNA typing, bloodstains were linked to a source by ABO typing and the characterisation of polymorphic blood enzymes and proteins
- This approach has now been supplanted by the newer DNA technology
- DNA analysis has allowed forensic scientists to associate blood and semen stains with a single individual
- Heredity and Paternity
- The transmission of hereditary material is accomplished by means of microscope units called genes, located on chromosomes
- Alternative forms of genes that influence a given characteristic (such as eye colour or blood type) are well known alleles
- Homozygous-similar alleles from both parents
- Heterozygous-different alleles from the parents
- Genotype- pair of alleles on the chromosomes
- Phenotype-physical expression of the allele
- [paternity testing has historically involved the ABO blood typing system along with blood factors other than ABO
- Currently, paternity testing has implemented DNA test procedures that can raise the odds of establishing paternity beyond 99 percent
- Testing for seminal stains
- Many cases sent to forensic laboratory involve sexual offences, making it necessary to examine exhibits for the presence of seminal stains
- Best way to locate and characterise seminal stain at the same time is to perform the acid phosphatase (an enzyme secreted into seminal fluid)colour test
- Purple colour indicates the acid phosphatase enzyme
- Semen can be identified either by the presence of spermatozoa or of p30, a protein unique to seminal plasma
- Forensic scientists can successfully link seminal material to an individual by DNA typing
- Rape evidence
- The rape victim must undergo a medical examination as soon as possible after assault
- At that time, the appropriate items of physical evidence, including clothing, hairs, and vaginal and rectal swabs, can be collected for subsequent laboratory examination
- All outer and undergarments should be carefully removed and packaged separately in paper (not plastic) bags
- Bedding,or objects upon which the assault took place, may also be carefully collected
- If a suspect is apprehended within 24 hours of assault, it may be possible to detect victims DNA on the males underwear or on a penile swab of the suspect
- Items routinely collected from the suspect include all clothing, pubic hair, head hair, penile swab, and blood sample or buccal swab for DNA typing
- The forceful physical contact between victim and assailant may result in a transfer of such physical evidence as blood, semen, saliva, hairs, and fibres
https://www.humbleisd.net/cms/lib2/TX01001414/Centricity/Domain/3959/blood%20typeKEY2015.docx
Ch. 10: DNA: the indispensable forensics science tool
- Introduction
- Portions of the DNA structure are as unique to each individual as fingerprints
- The gene is the fundamental unit of heredity
- Each gene is composed of DNA specifically designed to carry the task of controlling the genetic traits of our cells
- DNA is constructed as a very large molecule made by linkin a series of repeating units called nucleotides
- A nucleotide is composed of a sugar, a phosphorus molecule called a base
- The bases
- Four types of bases associated with the DNA structure:
- Adenine (A)
- Guanine (G)
- Cytosine ©
- Thymine (T)
- The bases on each strand ae properly aligned in a double helix configuration, which is two strands of DNA coiled together
- As a result, adenine pairs with thymine and guanine pairs with cytosine
- This concept is known as base pairing
- The order of the bases is what distinguishes different DNA strands
- Four types of bases associated with the DNA structure:
- DNA at work
- Dna directs the production of proteins which are made by combining amino acids
- The sequence of amino acids in a protein chain determine the shape and function of the protein
- Each group of three nucleotides in a dna sequence codes for a particular amino acid
- Example: G-A-G codes for amino acid glutamine, while CGT codes for alanine
- If nucleotide is changed,For example aT is a substituted for A and G-A-G becomes G-T-G the “wrong” amino acid is placed in the protein (in this case glutamine is replaced with valine)
- As a result, the protein may not function correctly, this is the basis for many diseases and health issues
- Normal red blood cell DNA sequence is CCT-GAG-GAG (proline, glutamate, glutamate) and sickle cell disease is CCT-GTG-GAT (proline, valine, glutamate) thus we see that a change is just one base can cause a major change in the production of a protein, which leads to malformed cells
- DNA replication
- DNA replicate itself prior to cell division
- DNA replication begins with the unwinding of the dna strand of the double helix
- Each strand is now exposed to a collection of free nucleotides that will be used to recreate the double helix, letter by letter, using base pairing
- Many enzymes and proteins such as dna polymerases, are involved with unwinding the dna, keeping the dna strands apart and assembling new dna strands
- Polymerase chain reactions (PCR) is a technique for replicating small quantities of dna or broken pieces of dna found at a crime scene outside living cell
- The ability to multiply small bits of dna now means that sample size is no longer a limitation in characterising dna recovered at a crime scene
- Recombination DNA
- Relies on the ability of certain chemicals, known as restriction enzymes, to cut dna into fragments that can later be incorporated into another dna strand
- Restriction enzymes Can be thought as a highly specialised scissors that cut a dna molecule when is recognises a specific sequence of bases
- Once a portion of the dna strand has been cut out with the aid of a restriction enzyme, the next step in the recombinant dna process is to insert isolated dna segment into a foreign dna strand, usually that of a bacterium
- As bacteria multiply rapidly, copies of altered dna are passed on to all descendants
- DNA typing
- Portions of the dna molecule contain sequences of bases that are repeated numerous times, known as tandem repeats
- To a forensic scientist, these tandem repeats offer a means of distinguishing one individual from another through dna typing
- Tandem repeats seem to act as a filler or spacers between the coding regions of DNA
- What is important to understand is that all humans have the same type of repeats,but there is tremendous variations in the number of repeats each of us have
- RFLP
- Length differences associated with relatively long repeating DNA strands are called restriction fragment length polymorphism and form the basis for one of the first dna typing procedures
- Typically, a core sequence consists of 15-35 bases and repeats itself up to one thousand times
- The key to understanding dna typing lies in the knowledge that numerous possibilities exist for the number of times a particular sequence of base letters can repeat itself on a dna strand
- Electrophoresis
- A technique of analogous to TLC is electrophoresis
- With electrophoresis, materials are forced to move across a gel coated plate under the influence of an electrical potential
- In this manner, substances such as DNA can be separated and characterised
- A technique of analogous to TLC is electrophoresis
- Positive RFLP test
- Once the DNA molecules have been cut up by restriction enzyme, the resulting fragments are sorted out by electrophoresis
- The smaller dna fragments will move at a faster rate on the gel plate than on the larger ones
- The fragments are then transferred to a nylon membrane in a process called southern blotting
- To visualise the RFLPSs the nylon sheet is treated with radioactive probes containing a base sequence complementary to the RFLPSs being identified (A process called hybridisation)
- Next, the nylon sheet is placed against X ray film and exposed for several days
- When the film is processed, bands appear where radioactive probes stick to fragments on nylon sheet
- Typical dna fragment pattern will show two bands (One RFLP from each chromosome)
- When comparing the dna fragment patterns of two or more specimens, one merely loos for a match between the band sets
- A high degree of discrimination can be achieved by using a number of different probes and combining their frequencies
- PCR testing
- Polymerase chain reaction is the outgrowth of knowledge gained from an understanding of how dna strands naturally replicate within a cell
- For the forensic scientist, PCR offers a distinctive advantage in that it can amplify minute quantities of dna many millions of times
- 1.the dna is heated to separate it
- 2.primers (short strands of dna used to target specific regions of dna for replication) are added , which hybridise the strands
- 3. DNA polymerase and free nucleotides are added to rebuild each of the separated strands
- This process is then repeated 25-30 times
- PCR and RFLP
- PCR technology cannot be applied to RFLP DNA typing
- The rflp strands are too long, often numbering in the thousands of bases
- PCR is best used with dna strands that are no longer than a couple hundred of bases
- PCR advantages
- One advantage in moving to short dna strands is that they would be expected to be more stable and less subject to degradation brought about by adverse environmental conditions
- LOng rflp strands tend to break apart readily under the adverse conditions not uncommon at crime scenes
- Pcr also offers the advantage in that it can amplify minute quantities, thus overcoming the limited sample size problem often associated with crime scene evidence
- Short tandem repeats
- The latest method of dna typing, short tandem repeat (STR) analysis, has emerged as the most successful and widely used dna profiling procedure
- STRs are locations on the chromosome that contain short sequences that repeat themselves within the dna molecule
- STRs serve as useful markers for the identification because they are found in great abundance throughout the human genome
- STR advantages
- STRs normally consist of repeating sequences of 3-7 bases in length and the entire stand of an str is also very short-less than 450 bases in length
- STRs are much less susceptible to degradation and may often be recovered from bodies or stains that have been subjected to extreme decomposition
- STRs are ideal candidates for multiplication by PCR, thus overcoming the previously mentioned limited sample size problem often associated with crime scene evidence
- Power of str
- What makes STRs so attractive to forensic scientists is that hundreds of different types of STRs are found in human genes
- The more str one can characterise the smaller the percentage of the population from which a particular combination of STRs can emanate
- This gives rise to the concept of multiplexing
- Using the technology of pcr, one can simultaneously Extract and amplify a combination of different STRs
- Standardising str testing
- Currently, us crime laboratories have standardised on 13 STRs for entry into a national database (CODIS)
- A high degree of discrimination and even individualisation can be obtained by analysing a combination of STRs (Multiplexing) and determining the product of their frequencies
- With STR, as little as 125 picograms of DNA is required for analysis
- This is 100 times less than that normally required for RFLP analysis
- Capillary electrophoresis
- The need to reduce analysis time and to automate sampling and data collection has led to the emergence of capillary electrophoresis
- Each end of the column is immersed in a reservoir of buffer liquid, which holds the electrodes
- The DNA sample is injected into one end of the column, and then with the electricity movement from one electrode to the other, the dna moves up and through the column
- As the dna peaks pass through the detector, they are recorded on a electropherogram
- Sex identification using STR
- With strs the focus of the attention here is the amelogenin gene located on both the X and Y chromosomes
- This gene is for tooth pulp, and is 6 base pairs shorter on the X chromosome than on the Y chromosome
- With the Y chromosome only part of the male genome, it makes it easier to direct the difference between male and female dna
- Mitochondrial dna
- Another type of dna used for individual characterisation s mitochondrial da (mtDNA)
- mtDNA is located outside the cells nucleus, and is inherited from the mother
- Mitochondria are structures found in all our cells used to provide energy that our bodies need to function
- S single mitochondria contains several loops of dna
- Mitochondrial dna typing does not approach str analysis in its discrimination power, and thus it is best reserved for samples, such as hair, which STR analysis may not be possible
- Forensics analysis of mtDNA is more rigorous, time consuming, and costly when compared to nuclear DNA analysis
- All individuals of the same maternal lineage will be indistinguishable by mtDNA analysis
- Two regions of mtDNA have been found to be highly variable, and the procedure known as sequencing is used to determine the order of basis pairs
- CODIS
- The most significant tool to arise from dna typing is the ability to compare dna types recovered from crime scene evidence to those of convicted sex offender and other convicted criminals
- CODIS (combined dna index system) is a computer software program developed by the fbi that maintains local, state, and national databases of dna profiles from convicted offenders, unsolved crime scene evidence, and profiles of missing persons
- CODIS currently contains about 470,000 profiles from unsolved cases
- Collection of DNA evidence
- Sources of dna
- Blood
- Semen
- Saliva
- Skin cells (touch DNA)
- Hair
- Bone
- Dna Is a powerful creator of physical evidence at crime scenes; for example, bottles, cans, glasses, cigarettes, bite marks, and envelopes
- Clothing from victim and suspect with blood evidence must be collected
- Dried blood is best removed from a surface by using sterile cotton swab lightly moistened with distilled water that is air dried before being placed in a swab box, then in paper or manila envelope
- Sources of dna
- Collection of DNA evidence
- Standard/reference DNA specimens must also be collected with a buccal swab (swab mouth and cheek)
- Packaging biological evidence
- Before collection of biological evidence begins, important that it be photographed and recorded on sketches
- Wearing disposable latex gloves while handling the evidence is required
- Gloves should be changed frequently
- Safety consideration and avoidance of contamination also call for the wearing of face masks, lab coat, eye protection, shoe covers, and possibly coveralls
- Packaging of biological evidence in plastic or airtight containers must be avoided bc the accumulation of the residual moisture could contribute to growth of dna destroying bacteria and fungi
- Minimise contamination
- Use disposable gloves
- Wear face mask while collecting evidence, lab coat, eye protection,and shoe covers
- Change gloves before handling each new piece of evidence
- collect the substrate control of possible subsequent laboratory examination
- Pick up small items of evidence such as cigarette butts and stamps with clean forceps
- Disposable forceps are to be used so that they can be discarded after a single evidence collection
- Always package each item of evidence in its own well ventilated container
- https://acutecaretesting.org/en/articles/methemoglobin
Ch. 11: Blood Splatter
- Crime scene reconstruction
- The method used to support a likely sequence of events by the observation and evaluation of physical evidence, as well as statements made by those involved with the incident, it is referred to as
- Crime scene reconstruction relies on the combined efforts of and personnel to recover physical evidence and to sort out the events surrounding the occurrences of a crime
- A crime scene investigator can bring special skills to the reconstruction of events that occured during commission of a crime
- For example, a laser beam is used to determine the search area for the position of a shooter who has fired a bullet through a window and wounded a victim
- Figure 4-1 a laser beam is used to determine the search area for the position of a shooter who has fired a bullet through a window and wounded a victims
- The bullet path is determined by lining up the victims bullet wound with the bullet hole present in the glass pane
- General bloodstain features
- Bloodstain patterns Deposited on and other relevant objects can provide valuable insights into events that occurred during the commission of a
- The crime scene investigator must remember that the and appearance of bloodstains and splatters may be useful for interpreting and constructing the events that produce the bleeding
- Bloodstain pattern interpretation may uncover
- The direction from which lood originated
- The angle at which blood struck the surface
- The location or position of victim at the time a bloody wound was inflicted
- Bloodstain attern interpretation may uncover
- The movement of a bleeding individual at the crime scene
- The approximate number of blows that struck a bleeding victim
- Approximate location of an individual delivering blows that produced a bloodstain pattern
- Effects of surface texture
- Surface texture and the stains shape size and location must be considered when determining a bloodstains direction dropping distance and angle of impact
- Surface texture is of paramount importance. In general the harder and less porous the surface the less spatter results
- Figure 4-2A
- A blood stain from a single drop of blood that struck a glass surface after falling 24 inches90
- Figure 4-2B
- A bloodstain from a single drop of blood that struck a cotton muslin sheet after falling 24 inches
- Directionality and angle of impact
- The direction of travel blood striking an object may be discerned because the pointed end of a bloodstain faces its direction to travel
- The impact angle of blood on a flat surface can be determined by measuring the degree of circular distortion
- At right angles the blood drop is circular a the angle decreases the stain becomes elongated
- Figure 4-4
- The higher pattern is of a single drop of human blood that fell 24 inches and struck hard smooth cardboard at 50 degrees
- On this drop the collection of blood shows the direction
- The lower pattern is of a single drop of human blood that fell 24 inches and then struck hard smooth cardboard at 15 degrees, on this drop the rail shows the direction
- Directionality and angle of impact
- Mathematically the angle of impact can be calculated by the equation and determining the inverse sine of A
- Sin A= Width of blood stain/length of blood stain
- Impact bloodstain spatter patterns
- Impact spatter occurs when an object impacts a source of blood
- Forward spatter Is projected outward and away from the source
- Back spatter, Also known as blowback spatter, is projected backward from the source
- Classifying impact spatter
- Using droplet size to classify impact patterns by velocity gives investigators insights into the general nature of a crime but cannot illuminate the specific events that produced the spatter pattern
- Low velocity spatter
- Drops with diameters of 4 mm or more normally produced by an applied force of up to 5 ft/sec
- Medium velocity spatter
- Drops with diameters from 1-4 mm with an applied force of 5-25 ft/sec
- High velocity spatter
- Drops with diameters of less than 1 mm from an applied force of 100 ft/sec or faster
- The classification of impact spatter as low medium and high velocity cannot illuminate the specific events that produced te stain size pattern
- In general one should use stain size categories very cautiously and for descriptive purposes only in evaluating impact spatter patterns
- Sources of blood spatter
- Impact spatter
- Cast off spatter
- Arterial spray spatter
- Figure 4-6A
- The action associated with producing impact spatter
- Figure 4-6B
- The action associated with the cast off spatter
- Figure 4-6C
- The action associated with producing arterial spatter
- Area of convergence
- The area of convergence is the point on a two dimensional lane from which the drops in an impact pattern originated
- This can be established by drawing straight lines through the long axis of several individual bloodstains following the line of their traits
- Figure 4-7
- Illustration of stain convergence on a 2d plane. Convergence represents the area from which the stains emanated
- Area of origin
- The area of origin of an impact bloodstain pattern is the area in a3d space from which the blood was projected
- This will show the position of the victim or suspect in space when the stain producing event took place
- The string method is commonly used at a crime scene to approximate the position of the area of origin using sound angles of impact of individual stains in the pattern
- Gunshot spatter
- Gunshot spatter is fine forward spatter from an exit wound and back spatter from an entrance wound
- The gunshot produces only back spatter if the bullet does not exit the body
- Depending on the distance from the victim that the gun was discharged, some back spatter may strike the gunman and enter the gun muzzle, this is called the drawback effect
- Cast off spatter
- A cast off pattern is created when a blood covered object fling blood in an arc onto a nearby surface
- This kind of pattern is commonly produced by a bloody fist or weapon between delivering blows
- The feature of the cast off pattern are affected by the size of the object, the amount of blood , ad the direction the object was moving
- By counting and pairing forward/backward patterns, one may determine the minimum number of blows was delivered
- Arterial spray spatter
- Arterial spray spatter is caused by an injury to the heart or a main artery and the pressure of the continued pumping
- The site of the initial injury to the artery can be found where the pattern begins with the biggest spurt
- The trail away from this point shows the victims movement
- The oxygenated blood spurting from the artery tends to be a brighter red colour than blood expelled from impact wounds
- Figure 4-11
- Arterial spray spatter found at crime scene where victim suffered injury to artery
- Expirated blood patterns
- An expirated blood pattern is created by blood that is expelled from the mouth or nose from an internal injury
- The presence of bubbles of oxygen in the drying drops or a lighter colour as a result of dilution in saliva can differentiate a pattern created by expirated blood
- The presence of expirated blood gives an important clue as to the injuries suffered and the events that took place at a crime scene.
- Void patterns
- A void is created when an object blocks the deposition of blood spatter onto a target surface or object, and the spatter is deposited onto the object or person instead
- The blank space on the surface or object may give clue as to the size and shape of the missing object or person
- Voids may be applicable for establishing the body position of the victim or assailant at the time of the incident.
- contact/transfer patterns
- A contact or transfer pattern is created when an object with blood on it touches one that does not have blood on it
- Simple transfer patterns are produced when the object makes contact with the surface and is removed without any movement of the object
- The size and general shape of object may be seen in simple transfer
- Other transfers known as swipe patterns may be caused by movement of the bloody object across a surface
- Figure 4-15
- A series of swipe patterns moving from right to left
- Flows
- Are made by drops of large amount of blood flowing by the pull of gravity
- Flow direction may show movement of object or bodies while the flow was still in progress oe after the blood dried
- Interruption of a flow pattern may indicate the sequence and passage of time between the flow and its interruption
- Pools
- A pool of blood occurs when blood collects in a level (not sloped) and undisturbed place
- Blood that pools on an absorbent surface may be absorbed throughout the surface and diffuse, creating a pattern larger than the original pool
- Considering the drying time of a blood pool can yield information about the timing of events that accompanied the incident
- Skeletonisation
- The phenomenon of skeletonisation occurs when the edges of a stain dry to the surface
- Tis usually occurs within 50 seconds of deposition of droplets and longer for larger volumes of blood
- After this time, if the bloodstain is altered through contact of a wiping motion, the skeletonised perimeter will be left intact
- Knowing the skeletonisation time, an investigator can determine the timing of movement or activity
- Drop trail patterns
- A drop trail pattern is a series of drops that are separate from other patterns, formed by blood dripping off an object or injury
- The stains form a kind of line or path usually made by the suspect after injuring or killing the victim or injuring themselves
- The pattern may show direction and speed of movement leaf to a discarded weapon or provide identification of the suspect by his or her own blood
- Documenting bloodstain evidence
- Investigator should not study and photograph each pattern and drop to record the locations of specific patterns accurately and to distinguish the stains from which lab samples were taken
- 2 techniques used to document bloodstain patterns are
- Grid method
- A grid of squares of known dimensions are set up over entire pattern
- perimeter ruler method
- Rectangular border of rulers is et up around each pattern and a smaller ruler is placed next to each stain
- Grid method
- Figure 4-19
- The grid method may be used for photographing bloodstain pattern evidence
- Figure 4-20
- The perimeter ruler method may be used for photographing bloodstain pattern evidence
- Interpreting bloodstain evidence
- Some jurisdictions have a specialist on staff who will decipher patterns either at the scene or from photographs at the lab
- However it is important that all personnel be familiar with patterns to record and document them properly for use in reconstruction
Ch 12: Trace evidence I- hairs and fibres
- Introduction
- Hair is encountered as physical evidence in a wide variety of crimes
- Although it is not yet possible to individualise human hair to a any single head or body through its morphology it still had value as physical evidence
- When properly collected and submitted to the laboratory accompanied by an adequate number of standard reference samples
- Morphology of hair
- Hair is an appendage of the skin that grows out of an organ known as the hair follicle
- The length of a hair extends from its root or bulb embedded in the follicle, continues into a shaft, and terminates at a tip end
- It is the shaft which is composed of 3 layers the cortex cuticle and medulla that is subject to the most intense examination by the forensic scientist
- Cuticle and cortex
- The cuticle is the scale structure covering the exterior of the hair
- The scales always point towards tip of hair
- Scale pattern is useful in species identification
- The cortex is the of the main body hair shaft
- Major forensics importance is the fact that it is embedded with pigment granules that impart hair with colour
- The colour shape and distribution of these granules provide the criminalist with important points of comparison among the hairs of different individual
- The cuticle is the scale structure covering the exterior of the hair
- Medula
- The medulla is a cellular column running through the centre of the hair
- The medullary index measures the diameter of the medullary relative to the diameter of the hair shafter
- For human the medulla generally occupies less than one third the diameter of the shaft while for animals it is generally one half or greater
- The medulla may be continuous, fragment, interrupted, or absent
- The presence of the medulla varies from individual to individual and even among hairs of a given individual
- Medulla also have different shapes depending on the species
- The medulla is a cellular column running through the centre of the hair
- Root
- The root and other surrounding cells in the hair follicle provide the tools necessary to produce hair and continue its growth
- When pulled from head some translucent tissue surrounding the hairs shaft near root may be found this is called follicular tag
- F=by using DNA analysis on the follicular tag the hair may be individualised
- Comparing strands
- Comparison microscope is indispensable tool for comparing the morphological characteristics of hair
- When comparing strands of human hair the criminalist is particularly interested in matching the colour, length and diameter
- F
- Scale structure medullary index and medullary shape Are particularly important in animal hair identification
- Other important features for comparing human hair are
- The presence or absence of medulla
- Distribution shape and colour intensity of pigment granules present in cortex
The most common request is to determine whether or not hair recovered at crime scene compares to hair removed from suspect
- However microscopic hair examinations tend to be subjective and highly dependant on the skills and integrity og analyst
- Questions forensics asks
- Cna the body area from which air originated be determined
- Can the racial origin of hair be determined
- Can age and sex of individual be determined
- Is it possible to determine if a hair was forcibly removed from body
- Are efforts being made to individualise human hair
- Is it possible to determine whether hair came from deceased individual
- Can DNA individualise a human hair
- Hair and DNA
- Recent major breakthroughs in DNA profiling have extended this technology to the individualisation of human hair
- The probability of detecting DNA in hair roots is more likely for air being examined in its anagen or early growth phase as opposed to its catagen (middle) or telogen (final)
- Often when hair is forcibly removes a follicular tag a translucent piece of tissue surrounding the hairs shaft near the root may be present
- This has proven to be a rich source of nuclear DNA associated with hair
- Hair and mitochondrial DNA
- mDNA can be extracted from hair shaft
- mDNA is found in cellular material located outside of the nucleus and it is transmitted only from the mother to child
- As a rule all positive
- Collection and preservation
- Hair samples are collected from victim of suspicious deaths during autopsy
- Types of fibres
- Natural fibres are derived in whole from animal or plant sources
- Wool mohair cashmere furs and cotton
- Man made fibres are manufactured
- Regenerated fibres are manufactured from natural raw materials and include raton acetate and triacetate
- Synthetic fibres Are produced solely from synthetic chemicals and include nylons polyesters and acrylics
- Natural fibres are derived in whole from animal or plant sources
- Qualitative vs quantitative
- Textile fibres provide many qualitative and quantitative traits or comparison
- Colour Is a good distinguishing characteristic dyes are used to make coloured textiles
- Applying statistical methods to trace evidence is difficult due to lack of frequency data can work for or against suspect
- How forensics scientist use fibres
- In investigation collection of fibres within 24 hours is critical
- Fibre evaluation can show such things as:
- Type of fibre: What is the composition of the fibre
- Its colour- does fibre match suspect clothes
- Possibility of violence: M9ore fibres associated with violence
- Location of suspects- how close can you place suspect to crime scene
- Point of origin/Carpet fibres, upholster from a car
- Sampling and testing
- Weaving spun fibres (yarns) together produces clothing and many textiles
- shedding f4om an article of clothing or a textile is the most common form of fibre transfer
- Natural fibres require only an ordinary microscope to find characteristic shapes and markings
- Infrared spectroscopy can reveal something of the chemical structure of other fibres that others may look very much alike
- If a large quantity of fibres is found some can be subjected to destructive tests such as burning them in flame Or dissolving then in various liquids
- Crimes Can be solved in this way by comparing fibres found on different suspects with those found at crime scene
- Fibre classification- natural fibres
- Woven wool textile
- Animal fibres (mae of proteins):
- From 3 sources: hair fur and webbing
- Hair is the most popular of animal fibres
- From 3 sources: hair fur and webbing
- Wool is from sheep (most popular) cashmere and mohair from goats angora from rabbits and hair from alpacas llamas and camels are used in textile
- Shimmering silk from caterpillar cocoons is longer and not easily as shed
- Caterpillars are reared in captivity each cocoon must be carefully unwound by hand
- Each caterpillar makes roughly 1500 – 3000 feet of silk fibre-it takes about 2000 caterpillars to make 1 lbs of silk- 150 caterpillars make one necktie
- Plant fibres (made of polymer cellulose):
- Can absorb water
- Some are amorphous, loose to arrangement that is soft, elastic, and absorbent
- Insoluble to water
- Are very resistant to damage from harsh chemicals
- Can only be dissolved from strong acids
- Can be common at crime scenes because they become brittle over time
- Seed fibres:
- Cotton is the most common and widely used seed fibre. Comes from cotton plant, easily woven and dyed(was at one time the most common export out of AZ)
- Kapok Fibre comes from trees native to africa and southeast asia
- Used mainly in life preservers- can hold 3x the weight of the preserver and doesn’t get waterlogged
- Fruit fibres:
- Coir iIs a coarse fibre obtained from a husk of coconuts
- The individual cells are narrow with thick walls, thus when woven together they are stronger than flax or cotton
- Good for doormats and baskets as it is waterproof
- Stem fibres:
- Hemp jute and flax are all produced from thick regions of plant systems
- Flax is most common, although not as popular due to cost
- Linen is smooth and used in plants jackets shirts and table cloths and bed sheets
- Jute fibres produce textile that is too coarse for garment and is used for ropes
- Hemp is similar to flax and has been used in asia for clothing
- Leaf fibres:
- Extracted from the leaves of abaca relative to banana leaves
- Can reach length of 10 feet
- Sisal, A desert plant, also provides fibres
- Used for making ropes, twines and netting
- Used around hay bales and deteriorates quickly
- Extracted from the leaves of abaca relative to banana leaves
- Mineral fibres:
- Are neither proteins or cellulose
- Fibreglass is a fibre form of glass
- Short weak brittle fibres used for building insulation. Early stick to skin causing itching and rash
- Asbestos is a naturally occurring fibre found in rocks with a crystalline structure
- Very durable used in pipe covering ceiling tiles floor tiles brake pads and fire resistant clothing
- Tied to mesothelioma cancer found in thin lining of the chest and lungs
- Plant fibres (Seed fruit stem and leaf fibres):
- Cotton from seed pods is the plant fibre most commonly used in textiles
- Coir from coconuts is durable
- Hemp just and flax (most common) from stems grow in bundles. Linen is a stem fibre made from fla
- Manilla and sisal from leaves deteriorate more quickly
- Mineral fibres:
- Fibreglass Is a fibrous form of glass
- Asbestos is a naturally occurring mineral with crystalline structure
- Fibre classification: synthetic fibres
- Until 19th century only plant and animal fibres were used to make clothes and textiles
- Half the products produced today are artificially produced
- Artificially produced fibres include rayon acetate nylon acrylics and polyester
- These fibres are made by joining many monomers together to form polymers
-
- Regenerated fibres (derived from cellulose)
- Rayon is most common-can imitate natural fibres but is strong
- Clelanese is cellulose chemically combined with acetate and is often found in carpets
- Polyamide nylon is cellulose combined with 3 acetate units, breathable, lightweight, and used in performance clothing.
- Synthetic polymer fibres:
- Petroleum is the basis of these fibres
- Monomers In large vats are joined together to form polymers. The fibres produced are spun together into yarns
- They have no internal structure, under magnification they show regular diameters
- Synthetic fibres are easily distinguished in a forensics lab
- Examples of synthetic polymer fibres:
- Polyester-found in polar fleece wrinkle resistant and not easily broken down by light or concentrated acid, added to natural fibres for strength
- Nylon easily broken down by light and concentrated acid;otherwise similar to polyester-introduced as synthetic silk
- Acrylic-inexpensive tends to ball easily and used as an artificial wood or fur
- Olefins-high performance ,quick drying, resistant to wear
- Regenerated fibres (derived from cellulose)
- Textiles and fabrics
- Weaving originated with basket making
- Weaving consists of arranging lengthwise threads (the wrap) and then woven crosswise threads(te weft) known as the weave pattern
- Weave patterns have names like:tabby twill and satin
- Tabby (or plain) is the simplest and forms a checkerboard patterns, has a 1/1 weave
- Twill Weaves are used in rugged clothing, hs 3/` weave (weft travels over 3 warp and then under 1)
- Satin Is a 3/1,4/1,5/1 or 6/1 weave pattern
- Weave pattern is one way that fabrics differ
- Thread count-# of threads packed together for any given amount of fabric (threads per inch)
- The price of sheets varies based on thread count and fibre tupe (all natural fibres and higher thread count increase cost)
- Types of fibres
- Polymers or macromolecules are synthetic fibres composed of a large number of atoms arranged in repeating units known as monomers
- Fibre evidence
- The quality of the fibre evidence depends on the ability of the criminalist to identify the origin of the fibre or at least be able to narrow the possibilities to a limited number of sources
- Obviously is the examiner is presented with fabrics that can be exactly fitted together at their torn edges, it is a virtual certainty that the fabrics were of common origin
- F=microscopic comparisons between questioned and standard/reference fibres are initially undertaken for colour and diameter characteristics using a comparison microscope
- Other morphological features that could be important in comparing fibres are:
- Lengthwise striations on the surface of the fibre
- The presence of delustering particles that reduce shine
- Cross sectional shape of fibre
- Compositional differences may exist in the dyes that were applied to fibres during manufacturing process
- Methods for fibre comparison
- The visible light microspectrophotometer is a convenient way for analysts to compare the colours of fibres through spectral patterns
- A more detailed analysis of the fibres dye composition can be obtained through a chromatographic separation
- Infrared spectrophotometry is a rapid and reliable method for identifying the generic class of fibres as does the polarising microscope
- Depending on the class of fibre each polarised plane of light will have a characteristic index of refraction
- Collection and preservation
- The investigators task of looking for minute stands of fibres often becomes one of identifying and preserving potential carriers of fibre evidence
- Relevant articles of clothing should be packaged carefully in separate paper bags
- It is necessary to remove a fibre from an object the investigator must use clean forceps place it in small sheet of paper fold and label paper and place paper packet inside another container
4pCh 13: metals paint and soil
- Trace elements
- Many manufactured products and even most natural materials contain small quantities of elements known as trace elements present in concentrations of less than 1 percent
- For the criminalist the presence of these trace elements is particularl;y useful bc they provide invisible markers that my establish the source of a material at least provide additional points for comparison
- Emission spectrograph
- An emission spectrograph vaporises and heats samples to a high temperature so that the atoms present in material achieve excited state
- Under these circumstances excited atoms emit light
- If the light is separated into its components one observes line spectrum
- Each element present in spectrum can be identified by its characteristic line frequencies
- Emission spectra can be matched line for line in comparison bt samples
- Origin of spectra
- Atom is composed of nucleus containing protons and neutrons with electrons found outside nucleus in electron orbitals
- The orbitals are associated with definite amount of energy called energy level
- Each element has its own set of characteristic energy levels at varying distances from nucleus
- Bc energy levels have fixed values an atom will absorb only definites value of energy which may come from heat or light
- This absorbed energy pushes the electrons into higher energy level orbitals an atom is now considered in an excited state
- Normally the electrons will not stay in this excited state for long and they will quickly fall back to original energy leek releasing energy in the form of light emission
- Emission spectroscopy collects and measures the various light energies given off by atoms
- The specific frequency of light absorbed or emitted can be determined by the relationship E=hf where E is energy difference bt 2 orbitals h s universal constant called Planck’s constant and f is frequency
- Bc each element has its own characteristic set of energy labels each will eit unique set of frequencies
- The absorptions of light by an atom, causing an electron to jumbo into higher orbital
- The emission of light by an atom caused by electron falling back to lower orbital
- Inductively coupled plasma
- InInductively coupled plasma emission spectrometry (ICP) the sample in the form of an is introduced into hot plasma creating charged particles that of characteristic wavelengths corresponding to the of elements present
- Two areas of forensic casework were ICP has been applied are the identification and characterisation of mutilated bullets and glass fragments
- Neutron activation
- A nuclear reactor is a source of neutrons that can be used for bombarding atoms causing some neutrons to be captured to produce radioactive isotopes Causing some neutrons to be captured to produce (atoms with same number of protons but different number of neutrons)
- Nuclear chemistry provides new tools for identifying and quantitating elements
- To identify radioactive isotope it is necessary to measure the energy of gamma rays emitted as radioactivity
- Neutron activation analysis measures the gamma ray frequencies of specimens that have been bombarded with neutrons
- This method provides highly sensitive and nondestructive analysis for simultaneously identifying and quantitating 20 to 30 trace elements
- forensic analysis has employed neutron activation on trace elements found in metals, drugs, paint, soil. Gun powder residue, and hair
- Since this technique requires access to a nuclear reactor, however, it has limited value to forensic analysis
- Fa detectors permits identification of the radioactive atoms present by measuring tr energies and intensity of the gamma rays emitted
- The neuron activation process requires the capture of a neutron by the nucleus of an atom. The new atom is now radioactive and emits gamma rays
- Paint
- Paint spread onto surface will dry into a hard film that can best be described as consisting of pigments and additives suspended in the binder
- One of the most common types of paint examined in the crime lab involves finished emanating from automobiles
- Automobile manufacturers normally apply variety of coatings to body of automobile
- These coatings may include electro coat primer, primer surface, basecoat and clearcoat
- Methods for paint comparison
- The wide diversity of automotive paint contributes to the forensic significance of an automobile paint comparison
- Paints can only be individualised if they have sufficiently complex layer structure
- Questioned and known specimens are best compared side by side under stereoscopic microscope for colour, surface texture, and colour layer sequence
- Pyrolysis gas chromatography and infrared spectrophotometry Are most invaluable techniques for distinguishing most paint binder formulations adding further significance to forensic paint comparison
- Solid materials such as paint may be heated or pyrolyzed to high temperatures so that they will decompose into numerous gaseous products to flow through GC column
- Crime labs are often asked to identify the make and model of a care from a small amount of paint and will make use of colour charts for automobile finishes pr the PDQ database
- Paint collection and preservation
- Paint chips are most likely found on or near persons or objects involved in hit and run incidents
- When transfer of paint occurs in hit and run scenarios uncontaminated reference paint must always be collected from undamaged area of car that is in close proximity to damaged area
- Paper druggist folds and glass/plastic vials make excellent containers for paint
- Paint smeared or embedded i garments or objects requires whole item to be packaged and sent to lab
- Tools used to gain entry into buildings or often contain traces of paint, requiring the tool be collected along with reference paint samples
- Soil
- The value of soil as evidence rests with its prevalence at crime scenes and its transferability between the scene and the criminal
- Most soils can be differentiated by their gross appearance
- A side by side visual comparison of colour and texture of soil specimens is easy to perform and provides a sensitive property for distinguishing soils that originate from different locations
- Comparison of soil
- In many forensic labs forensic geologists will characterise and compare the mineral content of soils
- Considering vast variety of mineral and rocks and possible presence of artificial debris in soil, the forensic geologist is presented with many points of comparison by two or more specimens
- Collection of soil
- standard/reference soils are to be collected at various intervals within a 100 foot radius of the crime scene as well as the crime site for comparison to the questioned soil
- Soil found on suspect such as that adhering to shoe or garments must be removed
- Instead each object should be individually wrapped in paper and transmitted to lab
;
Ch.14: forensic aspects of fire investigation
- Arson investigations often present complex and difficult circumstances to study due to the fact that:
- The perpetrator has thoroughly planned the act
- Is not present during act
- Destruction is so extensive
-
- The criminalists function is limited to detecting and identifying relevant chemical materials collected at the scene and reconstruction and identifying igniter mechanisms
- Fcriminalsit must establish a motive, modus operandi-or offender’s pattern of operation
- The criminalists function is limited to detecting and identifying relevant chemical materials collected at the scene and reconstruction and identifying igniter mechanisms
- The chemistry of fire
- chemically , fire is types of oxidation which is the combination of oxygen with other substances to produce new substances
- You can write the process as chemical equation: CH4 + 2O2 -> Co2 +2H2O
- To start fire, the minimum temperature needed to ignite fuel spontaneously known as ignition temperature must be reached
- The heat involved when substance burns is known as heat of combustion
- chemically , fire is types of oxidation which is the combination of oxygen with other substances to produce new substances
- Combustion:
- To ancient greek philosophers, fire was one of four basic elements from which all matter was derived
- Energy can be defined as ability or potential of a system or material to do work
- All oxidation reactions including combustion are examples of reactant where more energy is released than put in called exothermic reactions
- Some reactions require more energy to be put into them than are released, allied endothermic reactions
- Energy barrier (ignition temperature) must be hurdled before reactant such as methane and oxygen can combine with one another to form products of carbon dioxide and water as a vapour
- To ancient greek philosophers, fire was one of four basic elements from which all matter was derived
- The chemistry of fire
- An additional factor needed to explain fire is rate or speed at which oxidation takes place
- Fuel will achieve reaction rate with oxygen sufficient to produce flame only when it is in gaseous state
- Liquid burns when the temperature is high enough to vaporise it
- flash point)
- Solid must not be hot enough to decompose into gaseous products(pyrolysis)
- Glowing combustion or smouldering is burning at fuel air interface, such as that of a cigarette
- Flame point is lowest temperature that will allow liquid to produce flammable vapour
- Accelerants
- Bc fuel must be in vapour phase in order to start fire
- It can be relatively difficult to start and sustain fire, thus your campfire uses kindling and then bigger logs, and why arsonists use something else to het fires started
- Accelerants are fuels that are easily vaporised and support vaporisation
- Gasoline is most commonly used as it does not draw attention to person buying it such a kerosene, charcoal litres, and some paint thinners would
- Chemistry of fire
- Spontaneous combustion, which is rare, is the result of a natural heat producing process in poorly ventilated containers or areas
- When fire occurs, oxygen combines with fuel to produce noticeable quantities of heat and light
- To initiate and sustain combustion the following is required
- 1. A fuels must be present
- 2. Oxygen must be available in sufficient quantity to combine with fuel
- 3. Heat must be applied to initiate combustion and sufficient heat must be generated to sustain reaction
- 4. Chain reaction between fuel and oxygen
- Type of fires
- There are 3 types of natural fires
- Natural fires:
- Vast majority of natural fires are caused by lightning strikes
- There are few other natural fires as they tend to be from trying to stop natural gas leak and spark ignites gas into flame
- Accidental fires:
- May arise from any number of different sources
- Furnace fires, gas leaks, etc:
- In many of these cases the fire scene investigators would call in experts such as electricians, plumbers, or heating contractors
- Deliberate fires:
- Determinations of fire that may have new deliberately set involves a number of steps
- First fire investigator must eliminate possibility that it was natural or accidental
- Next they have to decide if the fire was arson or not
- Fire may be deliberately set but not with intent of hiding or destroying something
- Arson investigation can be added is accelerant is found to abe been used, or if there are multiple starting points for fire
- Other ways of classifying fires
- It is not only important to describe type of fire but also how started:
- Direct ignition: most basic cause of fire: involves spark or flame to ignite fuel. As simple as a match or difficult delay time start
- Electrical fires: electric appliances, wires, components, and connections are all capable of giving off spark or overheating
- Weather related fires: lightning strikes are very common and responsible for hundreds of forest fires annually
- Mechanical fires: a machine can overheat, such as a car where a lot of components are running hot near gasoline. also your clothes dryers with too much lint buildup in the vent can cause a fire to start
- It is not only important to describe type of fire but also how started:
- The basics
- Fuel will achieve a reaction rate with oxygen sufficient to sustain a fire only when its in the gaseous state
- Heat transfer
- The 3 mechanisms of heat transfer are conduction, radiation, and convection
- Conduction is the movement of heat through a solid object
- Radiation is the transfer of heat energy by electromagnetic radiation
- Convection is the transfer of heat energy by the movement of molecules within a liquid or gas
- The 3 mechanisms of heat transfer are conduction, radiation, and convection
- Investigation if fire scenes:
- Fire scenes are among the most difficult places to investigate
- Fires tend to destroy structures and thousands of gallons of water may be used to put out fire
- Structural integrity of structure may be compromised making it difficult and hazardous to go to
- Fire investigators have to be aware of causes of fires, burning patterns, how different materials react to fire, points of origin characteristics, how a fire normally and abnormally moves through structure, and potential use of fire suppression system
- Much like crime scene, fire investigator must take notes make general examination and try to find point of origin
- The fire scene
- Arson investigator needs to begin examining fire scene for signs of arson as soon as fire has been extinguished
- Experience shows that most arsons are started with petroleum based accelerants
- Accelerant is any material used to start or sustain a fire such as gasoline
- Necessity to begin an immediate investigation takes precedence even over requirement to obtain search warrant
- Experience shows that most arsons are started with petroleum based accelerants
- The search of fire scene must focus on finding fire origin which may be most productive in any search for accelerant or reaction device
- Telltale signs of arson include evidence of separate and unconnected fires, the use of streamers to spread the fire from one area to another, and evidence of severe burning found on floor, as opposed to ceiling due to flammable liquid
- Normally fire has tendency to move in upward direction and thus probable origin will most likely be the lowest point showing the most intense characteristics of burning (v pattern)
- Fortunately, combustible liquids are rarely entirely consumed during fire
- Thus collection of the remains of accelerant is possible
- It is most important to collect samples right away as accelerants can decompose evaporate or be lost due to clean up efforts
- Arson investigator needs to begin examining fire scene for signs of arson as soon as fire has been extinguished
- Points of origin
- Locating the points of origin has some general characteristics
- Low burning: fires usually start in low area of building-greater chance of major destruction
- V patterns: if near wall or in corner, smoke will make v shape pattern on wall where fire was started
- Wood charring: the depth of wood charring depends on intensity of heat, often most wood charring is near point of origin
- Material distortion: metal and glass may melt or distort owning to the high heat of starting fire
- Soot and smoke staining: amount of soot present in fire indicates point of origin
- Locating the points of origin has some general characteristics
- Indications of arson fire:
- There a numbers of factors that may be present that would indicate that fire was arson:
- Prescience of accelerant: if accelerant is present at/near point of origin the usually fire is arson, but not always
- Elimination of natural or accidental: this is necessary to determine that fire was arson
- Fire trails:in order for fire to travel rapidly in particular direction, fire trail must be employed
- multiple points of origin: pouring accelerant in multiple rooms of building to encase the building in fire more quickly is tactic used by arsonists
- There a numbers of factors that may be present that would indicate that fire was arson:
- Analysis of fire scene residue evidence
- The analysis of fire scene residue consists of 2 major steps:
- 1st: need to isolate accelerant, usually ignitable liquid, usually involves and extraction but can be direct capture
- 2nd determine the nature of the accelerant residue, this involves the determination of accelerant. Gas chromatography is universally used to determine type of hydrocarbon
- Collection
- at the suspected point of origin of a fire, ash and soot, along with porous materials that may contain excess accelerant, should be collected and stored in airtight containers, leaving an airspace to remove samples
- Traces of flammable liquid residue may be located with vapour detector (sniffer)
- It is important that sampling of similar but uncontaminated control specimens be collected
- Search for igniters, such as matches, electrical sparking device, or parts of Molotov cocktail must also be conducted
- Packaging evidence must be done in airtight container so possible residues are not lost due to evaporation
- Analysis of flammable residue
- Bf accelerant residues can be analysed, they must be collected. There are two techniques for doing this:
- Headspace technique: heat container holding fire debris which releases accelerant into headspace of container; use syringe to pull the residue up through a port in the top of container
- Charcoal coated strip is placed inside the container, which is heated to -60 c. Gas particles are attracted to carbon strip which gets removed, washed with solvent (carbon disulfide) then solution is transferred into gas chronograph machine
- Bf accelerant residues can be analysed, they must be collected. There are two techniques for doing this:
- Gas chromatography
- In lab the gas chronograph is most sensitive and reliable instrument for detecting and characterising flammable residues
- The vast majority of arsons are initiated by petroleum distillates such as gasoline and kesone due to their accessibility and their low ignition temperature
- Interpretation of fire scene evidence
- There are 2 major goals in fire scene investigations:
- 1sr: determining type of fire-accidental, natural, or deliberate
- 2nd: if fire was deliberately set
- When viewing an accelerant with CG remember that there is a high probability that there will be contaminated in the sample and that all of accelerant that is left is the high temperature
- The term weathering is used to describe the degradation of an accelerant due to heat or other environmental factors
- There are 2 major goals in fire scene investigations:
- Gas chromatography
- Gas chromatography separates the hydrocarbon components and produces a chromatographic pattern characteristic of a particular petroleum product. Gas chromatograph is the most sensitive and reliable instrument for detecting and characterising flammable residues
- By comparing select gas chromatographic peaks recovered from fire scene debris to known flammable liquids, a forensic analyst may be able to identify accelerant used to initiate fire
Ch 15: forensic investigation of explosion
- Oklahoma City bombing
- At the time is was the largest act of mass murder in us
- Alfred p Murrah federal building had an open parking garage structure that was tall enough to drive truck into
- April 19 1995 the driver of truck casually walked away, minuted later the truck exploded into fireball that destroyed majority of 9 story building, killing 168 people
- Old 1977 mercury marquis was seen leaving scene just prior to
- F
- Rental truck was quickly located at scene and was traced back to local agency where the name of Robert king-alias floor Timothy my veigh was used to rent truck
- F
- F
- My veigh was sentenced to death by lethal injection and nichols is serving life in prison without possibility of parole
- Your turn
- Explosions and explosives
- Black powder
- Chlorate mixtures:
- The only ingredients required for low explosive are fuel and good oxidising agent
- Potassium chlorate mixed with sugar produces popular and accessible explosive mix, when contained it explodes with force equivalent to stick of 40% dynamite
- Chlorate mixtures may also be ignited by the heat generated from chemical reaction (sulfuric acid and sugar chlorate mixture)
- Gas air mixtures
- In this instance we are referring to natural gas such as methane
- Mixtures of gases and oxygen only burn within certain concentration range
- For methane the range is between 5.3 and 13.9 otherwise there is too little gas or too much oxygen to support combustion
- If concentration is on high end rich when it explodes not all gas will be consumed first time
- As air rushes in the residual hot gas produces fire that is more destructive than explosion
- If concentration is on low side lean then explosion won’t be accompanied by fire afterwards
- High explosives
- An explosion with velocity of detonation greater than 1000 metres per seconds
- Includes TNT, PETN, and RDX are between 1000 and 8500 mps
- These types do not need to be contained; classified into two groups, primary and secondary, based on their sensitivity to heat, shock, or friction
- Primary explosives are ultra sensitive to heat shock or friction and provide the major ingredients found in blasting caps or primers used to detonate other explosives (chain reaction)
- Secondary explosives are relatively insensitive to heat shock or friction and will normally burn rather than detonate if ignited in small quantities in the open air
- Secondary explosives
- This group comprises the majority of commercial and military blasting, such as dynamite, TNT (trinitrotoluene) , PETN (pentaerythritol tetranitrate) , RDX (cyclotrimethylenetrinitramine) , and tetryl (2,4,6-trinitrophenylmethylnitramine)
- Dynamite
- Humankind’s irony is that the prize mo
- st symbolic for humanity’s search for peace the nobel peace prize is named for the man that developed dynamite
- 1867 swedish chemist alfred nobel searching for way to desensitise nitroglycerin mixed it with variety of diatomaceous earth
- Later used pulp to absorb nitroglycerin which is what dynamite is today
- This is used when quick shattering is the desired outcome, all straight dynamite is rated by strength such as 40 or 60%
- The strength of explosion is not proportional rto they ratings
- 605 is only 1 ½ x as strong as 20% not the 3x as would be expected
- Ammonium nitrate explosives
- In recent years nitroglycerin based dynamite has disappeared from the industrial explosive market and has been replaced by ammonium nitrate based explosives (ex. Water gels, emulsions, and ANFO explosive)
- Secondary explosives must be detonated by primary explosive
- Water gels have consistency of gel toothpaste and are used for blasting in wet conditions
- Emulsions differ from gels in that they consist of two phases, oil phase and water phase. Droplet of supersaturated solution of ammonium nitrate is surrounded by a hydrocarbon serving as fuel. Typical emulsion consists of water, one or more inorganic nitrate oxidisers oil or ceramic spheres known as microspheres or balloons
- Ammonium nitrate soaked in fuel oil is an explosive known as ANFO. these are inexpensive and safe to handle and used in mining industry.is a typical component of homemade bombs (used in oklahoma city bombing)
- Military high explosives
- In many countries outside us the accessibility of military high explosives to terrorist organisations make them very common constituents of homemade bombs
- RDX is most popular and powerful of military explosives often encountered in the form of pliable plastic known as C-4
- Military dynamite does not contain nitroglycerin but a mixture of RDX and TNT. tnt is rarely encountered in bombings in us
- PETN is used by military in TNT mixtures for small calibre projectiles and grenades
- Detonation cord: cordlike explosive containing core of high explosive material usually pet also called primacord
- Detonators: unlike low explosives, bombs made of high explosives must be detonated by initiating an explosion. In most cases detonators are blasting caps which can be initiated by safety fuse or electrical current
- High explosives
- Triacetone triperoxide (TATP) id homemade explosive that has been used by terrorist organisations
- TATP can be made by combining acetone and peroxide in presence of and acid
- Its an existence has led to the banning of most liquids on commercial aircraft
- collection and analysis
- Entire bomb site must be systematically searched with great care given to recovering any trace of a detonating mechanism or any other item foreign to explosion site
- Objects located at or near origin of the explosion must be collected for laboratory examination
- Detecting and recovering evidence
- Often crater is located at the origin and loose soil and other debris must be preserved from its interior for lab analysis
- In pipe bombs, particles of materials can be found embedded to pipe
- One approach for screening objects for the presence of explosive residues in the field or laboratory is the ion mobility spectrometer (IMS)
- Collecting and packaging
- Preliminary identification of explosive residue using IMS can be made by noting the time it takes the explosive to move through tue, confirmatory test must follow
- All material collected for examination by lab must be placed in sealed air tight containers and labelled with all pertinent information
- Debris and articles collected from different areas are to be packaged in separate air tight containers
- It has been demonstrated that some explosives can diffuse through plastic and contaminate nearby containers
- Back at the lab
- Typically in lab debris collected at explosion scenes will be examined microscopically for unconsumed explosive particles
- Recovered debris also be thoroughly rinsed with organic solvents such as acetone
- Water gels and the like should be rinsed with water
- Screening and confirmatory test
- Once collected the acetone extract is concentrated and analysed using colour spot tests, thin layer chromatography (TLC), high performance liquid chromatography and gas chromatography/ mass spectrometry
- HPLC is highly sensitive and good for trace elements as well as high explosives
- Back at lab
- Confirmatory identification tests may be performed on unexploded material either by infrared spectrophotometry or x ray diffraction
- X ray diffraction
- X ray diffraction is applied to study of solid crystalline materials
- As x ray penetrate crystal a portion of beam is reflected by each of atomic planes
- As reflected beams leave the crystals planes they combine with one another to form series of light and dark bands known as diffraction pattern
- Every compound is known to produce its own unique diffraction pattern thus giving analysts a means for fingerprinting crystalline compounds
Ch 15: fingerprinting
- History
- The first systematic attempt at personal identification was devised by french police expert, alphonse bertillion
- The bertillon system relied on detailed description of subject combined with full length and profile photographs and system of precise body measurements (11) called anthropometry
- The chinese used fingerprints to sign legal documents as far back as 3,000 years ago
- Henry fauld in 1880 suggested that skin ridge patterns could b used to identify criminals
- He even offered his own services and expenses to set up fingerprint bureau at scotland yard but was denied in favour of bertillon system
- In 1892 francid galton published his classic textbook fingerprints
- In this book he discussed the anatomy of fingerprints and the three patterns and suggested methods for recording them
- He also demonstrated that no two fingerprints were alie and did not change over the course of one’s life time-most important facts
- Fingerprinting
- At galtons insistence the british government adopted fingerprinting as a supplement to bertillon system
- The next step was the creation of classification systems capable of filing many thousands of prints in logical and searchable sequence
- Dr juan vucetuch devised a classification system still used in most spanish speaking countries while sir edward henry devised another classification system used in most english speaking countries
- In 1903 when the bertillon system could not distinguish between two men (one will west and the other william west) it was fingerprinting that clearly distinguished them
- The new york city civil service commision started in 1901 using fingerprints to identify their officers and other civil servants;the training of american police by scotland yard representatives at 1904 world’s fair, fingerprinting began began to be used in earnest in all major us cities (thanks to ill west)
- Fingerprinting principles
- Fingerprints are a reproduction of friction skin ridges found on palm side of the fingers and thumbs
- The basic principles underlying the use of fingerprints in criminals investigators are that
- 1: a fingerprint is an individual characteristic because no two fingerprints have yet been found to possess identical ridge characteristics
- 2: a fingerprint will remain unchanged during an individual’s lifetime
- 3: fingerprints have general ridge patterns that permit them to be systematically classified
- Principle one
- Mathematically the probability for the existence of two identical fingerprint patterns in the world’s population is extremely small (64 billion different possibilities)
- Besides theoretical calculations of the millions upon millions of individuals who have had their prints classified no two fingerprints have been found to be identical
- The individuality of a fingerprint is not determined by its general shape or pattern but by the careful study of its ridge characteristics known as minutiae
- It is the identity number, and relative location of these minutiae that imparts individuality to a fingerprint
- There are as many as 150 minutiae on the average finger
- After a three year study it was determined that ni valid basis exists for requiring a predetermined minimum number of friction ridge characters which must be present in two impressions in order to establish positive identification
- In judicial proceeding an expert must demonstrate a point by point comparison in order to prove the identity of an individual
- Principle two
- The epidermis is the outer layer of the skin, the dermis is the inner layer of skin
- The dermal papillae is the layer of cells between the epidermis and dermis which is responsible for determining form and pattern of ridges on the surface of the skin
- Once the dermal papillae develop in the human foetus, the ridge patterns will remain unchanged throughout life except to enlarge during growth
- One cannot completely alter their fingerprints as the new scars only make for new ways to identify you
- Each skin ridge is populated with pores leading to sweat glands from which perspiration is deposited onto skin
- Pmce the finger touches a surface, perspiration, along with oils that may have been picked up by touching the hairy portions of the body, is transferred onto that surface, leaving the fingers ridge pattern (a fingerprint)
- Principle three
- All fingerprints are divided into three classes on the basis of their general pattern: loops, arches, and whorls
- 60-65% of population have loops, 30-35% have whorls, 5% have arches
- Loops
- A loop must have one or more ridges entering from one side of the print, recurving, and exiting from the same side
- If loop opens towards little finger it is called ulnar loop
- If loop opens towards thumb it is called radial loop
- A loop must have one or more ridges entering from one side of the print, recurving, and exiting from the same side
- Whorls
- Whorls are divided into four groups: plain, central pocket loop, double loop, and accidental
- A plain whorl and a central pocket loop have at least one ridgethat makes a complete circuit
- Double loop is made up of two loops combined into one fingerprint
- An accidental either contains two or more patterns or is a pattern not covered by the other categories. Hence an accidental may consist of a combination loop and plain whorl or loop and tented arch
- Arches
- Arches, the least common of three patterns, are divided into two groups: plain arches and tented arches
- The plain arch is form by ridges entering from one side of the print rising and falling and exiting on the opposite side (like a wave)
- The tented arch is similar to the plain arch except that instead of rising smoothly at centre there is a sharp upthrust or spike or the ridge meet at an angle that is less than 90 degrees
- Ace v
- A four step process to identify and individualise a fingerprint:
- 1. Analysis
- 2. Comparison: compare the questioned print at 3 levels
- 3. Identify
- 4. Verification: examiners conclusion is confirmed by second examiner
- Level one: encompasses the general ridge flow and pattern configuration
- Level 2 includes locating and comparing ridge characteristics or minutiae
- Level three includes the examination and location of ridge pores, breaks, creases, scars, and other permanent minutiae
- A four step process to identify and individualise a fingerprint:
- Primary classification
- Fingerprint classification systems are based on knowledge of fingerprint pattern classes
- First, fingers are paired up, placing one finger in the numerator of a fraction and the other in the denominator (part of original henry system, which used #s and letters)
- R. index/R. thumb , R. ring/R. Middle, L thumb/ R. little, L middle/ L index, L little/ L. Ring
- The presence or absence of the whorl pattern classification is the basis for the determination of primary classification
- If a whorl pattern is found on any finger of the first pair, it is assigned value of 16 the second pair an 8 the third pair a 4 on the fourth pair a 2 and on the last pair a 1
- Any finger having a loop or arch is assigned a 0
- After values for all 10 fingers are obtained they are totalled and a 1 is added to both the numerator and denominator just in case there are no whorls
- The fraction thus obtained is the primary classification
- Approximately 25 percent of the population falls into 1/1 category that is all their fingers either have loops or arches
- A fingerprint classification cannot itself unequivocally identify an individual, it will merely provide fingerprint examiner with number of candidates all of whom have indistinguishable set of print in systems file
- It will merely provide the fingerprint examiner with a number of candidates, all of whom have an indistinguishable set of prints in systems file
- AFIS
- The heart of Avis tech is the ability o computer to scan and digitally encodings
- AFIS aids in and fingerprints by converting the image of a fingerprint into digital minutiae that contain data showing ridges at their points of termination (ridge endings) and their branching into two ridges (bifurcations)
- When the search is complete (a computer can make thousands of comparisons per second) the computer produces list of file prints that must be examined by a trained fingerprint expert
- Latent prints
- Once the finger touches a surface body perspiration or oils present on the finger ridges are transferred to that surface leaving an impression
- Prints deposited in this manner are invisible to the ye and are commonly referred to as latent or invisible fingerprints
- Visible prints
- Visible prints are made when fingers have touched surface after ridges had been in contact with coloured material such as blood paint grease or ink
- Plastic prints are ridge impressions left on soft material such as putty wax soap or dust
- Locating visible or plastic prints at the crime scene normally presents little problem to the investigator because these prints are usually distinct an visible to the eye
- Latent prints
- Deposited on hard non absorbent surface (for example glass mirror tile and painted wood) are preferably developed by the applications of a powder
- Prints on porous surface (that is papers cardboard and cloth) generally require treatment with a chemical
- Examiners use various chemical methods to visualise latent prints on porous surfaces such as ironing fuming ninhydrin physical developer
- Superglue fuming develops latent prints on nonporous surfaces metals electrical tape leather and plastic bags
- Development occurs when when fumes from glue adhere to print
- Detecting prints
- A device called the reflected ultraviolet imaging system (RUVIS) can aid in the detection of latent fingerprints without chemicals or powder
- Once located crime scene investigators can develop print in most appropriate fashion
- Powders available in a variety of colours can be applied with a brush or magnetic wand and adhere to the perspirations and body oils of print
- Iodine fuming involves heating iodine with crystals that cause vapes which combine with latent prints to make them visible
- Iodine prints are not permanent we will fade making it necessary to photograph it immediately
- Ninhydrin reacts chemically with trace amounts of amino acids present in latent prints to produce purple blue colour
- Physical developer is a silver nitrate based reagent used to develop prints when other chemical methods are ineffective
- Super Glue is approximately91
- Superglue fuming can be accomplished by using either a fuming chamber for up to six hours or a handheld wand that heats small cartridge containing cyanoacrylate
- F
- Fingerprints are treated with chemicals that would induce fluorescence when exposed to lasers, or high intensity sources (alternate light sources such as quartz halogen, xenon arc, or indium arc light sources
- F
- Transporting prints:
- F
- Prints on large immovable objects that have been developed with a powder can best be preserved by lifting with broad adhesive tape
- F
- Digital imaging
- F
- With the help of digital imaging software, fingerprint, which are not always in perfect condition, can ow be enhanced for most accurate and comprehensive analysis
- Is compare function that places two images side by side and allows examiner to chart common features on both images simultaneously
